CRISPR/Cas技术及其在作物遗传育种中的应用

CRISPR/Cas Technology and Its Application in Crop Genetic Breeding

简要概述了ZFN、TALEN和CRISPR/Cas系统3种基因组编辑技术的工作原理,介绍了CRISPR/Cas技术在功能基因组学研究、候选基因功能分析(验证)、分子育种方面的应用及其在作物产量、品质、抗病性及水稻雄性不育系创制上的研究进展,提出该项技术在植物遗传育种应用中的新思路及安全性等研究方向。

This paper makes a brief overview of the working principles of the three genome edits of ZFN,TALEN and CRISPR/Cas systems,and introduces the application of CRISPR/Cas technology in functional genomics research,candidate gene function analysis (validation),and molecular breeding.The research progress is expounded in crop yield,quality,disease resistance and creation of rice male sterile line.