代谢组学法研究转录因子SlNAC4对番茄果实代谢产物的影响

Metabonomic Study on the Effect of Transcriptional Factor SlNAC4 on Tomato Fruit Metabolites

以SlNAC4基因沉默番茄和野生型番茄为研究对象,采用高效液相色谱-串联高分辨率质谱仪对破色期番茄果实代谢产物进行代谢组学分析,探究转录因子SlNAC4对果实成熟中代谢产物的影响。利用XCMS软件和inhouse标准品、HMDB、KEGG等数据库对化合物进行定性分析,用metaX软件进行定量及差异代谢产物的筛选。对两组番茄代谢组学分析结果显示,既能与数据库物质的一级离子m/z匹配也能与数据库物质的碎片离子(二级)m/z匹配到的差异代谢物质共有67种,包括氨基酸衍生物及二肽类(19种)、维生素类(7种)、糖类及其衍生物(4种)、核酸及其衍生物(11种)、生物碱类(4种)、香精香料(4种)及有机酸(9种)等。其中有29种化合物含量上调,38种含量显著下降,表明番茄转录因子SlNAC4影响了番茄果实成熟过程中的代谢。

In this study, SlNAC4 gene-silencing and wild-type tomato fruit were used for metabonomic investigation of tomato fruit metabolites at the breaking stage by using high performance liquid chromatography coupled to high-resolution tandem mass spectrometer(HPLC-HRMS/MS) in order to evaluate the effect of transcriptional factor SlNAC4 on tomato fruit metabolites during its ripening. The compounds were qualitatively analyzed by comparison of their mass spectral data with those in HMDB and KEGG databases as well as those of in-house reference standards using the XCMS software. The quantitative analysis and differential metabolite screening were carried out using metaX software. The results showed that a total of 67 differential metabolites that could match both the mass spectral and tandem mass spectral data(m/z) were found,including 19 amino acid derivatives and dipeptides, 7 vitamins, 4 carbohydrates and their derivatives, 11 nucleic acids and their derivatives, 4 alkaloids, 4 fiavors and 9 organic acids, 29 of which were up-regulated whereas the rest were significantly down-regulated, indicating that SlNAC4 transcriptional factors affects tomato fruit metabolism during the ripening process.