沉默EZH2基因抑制胃癌细胞增殖及促凋亡研究

Silencing EZH2 gene inhibits proliferation of gastric cancer cell and promotes its apoptosis

目的探讨沉默胃癌细胞株MGC803中EZH2基因对细胞增殖及凋亡的影响。方法采用小干扰RNA(siRNA)沉默胃癌细胞中的EZH2基因并用实时荧光定量PCR(qRT-PCR)及蛋白质印迹(Western blot)验证沉默效率,MTT法评估细胞增殖情况,运用Western blot分析凋亡相关蛋白——Bcl-2及Bax,流式细胞仪检测细胞的凋亡变化。结果胃癌细胞中的EZH2基因被成功沉默;沉默组细胞的增殖情况相较于正常组和阴性对照组明显降低,而凋亡率相较于正常组和阴性对照组则明显升高,同时,沉默组中的Bcl-2蛋白表达量降低而Bax蛋白表达则显著升高,差异均有统计学意义(P<0.05)。结论沉默胃癌细胞中的EZH2基因能抑制该肿瘤细胞的增殖并促进其凋亡。

Objective To explore the effect of siliencing EZH2 on proliferation and apoptosis in gastric cancer cell line MGC803.Methods Silencing EZH2 was performed by siRNA transfection,qRT-PCR and Western blot were used to verify the efficienly of silence.The effect of proliferation and apoptosis were detected by MTT assay and flow cytometry,respectively.Western blot was used to indicate the expression of Bcl-2 and Bax.Results EZH2 gene was successfully silenced in gastric cancer cells.The proliferation of silencing group was significantly lower than that of normal group and negative control group,the apoptosis rate was significantly increased compared with the normal group and negative control group,while the protein expression of Bcl-2 in siRNA group was significantly down-regulated compared with the control and negative control group but Bax was the opposite with statistically significant difference (P<0.05).Conclusion Silencing EZH2 gene can inhibit proliferation and promotes apoptosis in gastric cancer cells.