阿司匹林对耐药幽门螺杆菌的体外影响

Effect of aspirin on resistant helicobacter pylori in vitro

目的:探讨阿司匹林(aspirin)对幽门螺杆菌(helicobacter pylori,HP)克拉霉素耐药性的影响,并分析其可能的作用机制。方法:通过克拉霉素药物浓度倍增的方法诱导体外培养的HP26695获得HP26695克拉霉素耐药菌株。采用琼脂稀释法检测阿司匹林对耐药菌的最低抑菌浓度(minimum inhibitory concentration,MIC),而后通过低浓度的阿司匹林持续培养耐药菌30d,检测其对克拉霉素耐药性的变化,并采用实时荧光定量PCR检测外排泵系统基因hefABC的表达情况。结果:HP26695利用克拉霉素药物浓度倍增法成功诱导出HP26695克拉霉素耐药菌株,其MIC稳定为8μg/mL。阿司匹林可剂量依赖性地抑制HP26695克拉霉素耐药菌的体外生长。低剂量阿司匹林体外持续作用30d可降低克拉霉素对HP26695克拉霉素耐药菌的MIC,增加耐药菌的敏感性;但并不影响耐药菌外排泵基因hefABC的表达。结论:阿司匹林对HP26695克拉霉素耐药菌具有一定的生长抑制作用,并可提高其对克拉霉素的敏感性;但该作用与耐药菌外排泵基因hefABC的表达无关。

Objective:To evaluate the effect of aspirin on the clarithromycin resistance of helicobacter pylori (HP) and explore its possible mechanism.Methods:HP26695 clarithromycin resistant strain was obtained by multiple concentration of clarithromycin stimulation to HP26695 standard strain.Agar dilution method was used to detect the minimum inhibitory concentration (MIC) of aspirin to HP26695 clarithromycin resistant strain.After clarithromycin resistant HP was cultured in medium with low concentrate aspirin for 30 days,its resistance to clarithromycin was detected,and the gene expression of efflux pump system hefABC was measured by quantitative real-time PCR.Results:HP26695 clarithromycin resistant strain was successively obtained with multiple concentration of clarithromycin stimulation,and its MIC was 8 μg/mL.After incubated with low concentrate aspirin for 30 days,the MIC of HP26695 clarithromycin resistant strain was declined and its sensitivity to clarithromycin was increased,while the gene expression of efflux pump system hefABC in clarithromycin resistant HP hadn’t changed.Conclusion:Aspirin could inhibit the growth of clarithromycin resistant HP at some point,and enhanced its sensitivity to clarithromycin,while this action was not associated with the gene expression of efflux pump system hefABC.