摘要
建立了丹敏停胶囊中丹参酮ⅡA的高效液相色谱检测方法。色谱柱:WondaSil C_(18)(5μm,4.6 mm×250 mm);流动相:甲醇-水(80∶20);检测波长:270 nm;流速:1.0 mL/min;柱温:25℃。丹参酮ⅡA在0.017 1～0.274 2μg进样量与色谱峰面积呈良好的线性关系;平均回收率为99.87%,RSD为0.57%。该研究建立的丹敏停胶囊质量控制方法简单、结果稳定、重复性良好,可以用于控制该制剂的质量。
A method for the determination of tanshinone ⅡA in Danminting capsules by high performance liquid chromatography was established. Chromatographic column:WondaSil C18(5μm,4.6 mm×250 mm);mobile phase:methanol-water(80∶20);detection wavelength was 270 nm;flow rate was 1.0 mL/min;column temperature was 25 ℃.In the range of 0.017 1-0.274 2μg, the injection quantity of tanshinone ⅡA showed a good linear relationship with the peak area of chromatography, the average recovery rate was 99.87% and RSD was0.57%. The quality control method of Danminting capsule established in this study is simple,stable and reproducible. It can be used to control the quality of Danminting capsule.
作者
慕佳冶
朱昆
王颖
汪旭
赵亚男
李忻
MU Jia-ye;ZHU Kun;WANG Ying;WANG Xu;ZHAO Ya-nan;LI Xin(China-Japan Union Hospital of Jilin University,Department of Pharmacy,Changchun 130033,China;Changchun Central Hospital,Preparation Room,Changchun 130024;Jilin Institute of Food and Drug Control,Changchun 130033,China)
出处
《特产研究》
2019年第2期89-90,104,共3页
Special Wild Economic Animal and Plant Research
