快速心房起搏刺激联合卡巴胆碱建立小鼠房颤/房扑模型

Rapid atrial pacing stimulation combined with carbachol to establish atrial fibrillation/atrial flutter model in mice

目的在C57B6N小鼠中利用快速心房起搏联合卡巴胆碱技术建立房颤/房扑模型。方法选取14周龄C57B6N雄性小鼠62只,体重(25±3)g,连接肢体导联记录体表心电图,经颈静脉插入八电极导管到右心记录心内心电图,各随机选择20只小鼠作为假手术组和两次单纯心房起搏组,余22只小鼠行心房猝发(Burst)刺激起搏作为单纯心房起搏组,联合诱导组小鼠经球后静脉注射卡巴胆碱(50 ng/g)后重复上述心房Burst起搏。分析心电图电生理参数和房颤/房扑持续时间。应用SPSS 22.0统计软件进行分析。结果假手术组(20只)未观察到自发室上性心律失常;两次单纯心房起搏组(20只)有4只(20.0%)小鼠诱导出房颤/房扑;单纯心房起搏组中,4/22只(18.2%)小鼠发生房颤/房扑,平均持续时间为(10.7±13.3)s,两次单纯心房起搏组与单次单纯心房起搏组有诱导房颤/房扑成功率无统计学差异(P>0.05);联合诱导组中,13/20只(65.0%)小鼠发生房颤/房扑,平均持续时间为(44.6±171.7)s;单纯心房起搏组和联合诱导组诱导成功率及平均持续时间的差异有统计学意义(P<0.05)。结论通过快速心房起搏联合卡巴胆碱技术,可以建立一种简单、有效的小鼠房颤/房扑模型。

Objective To establish atrial fibrillation/atrial flutter(AF/AFL)model in C57B6N mice using rapid atrial pacing combined with carbachol.Methods 62 male C57B6N mice,weighing(25±3)g.A surface electrocardiogram(ECG)was recorded from surface limb leads.20 mice were randomly and respectively selected as sham operation group and twice simple atrial pacing groups.An octapolar electrode catheter was inserted via jugular vein cutdown approach for simultaneous atrial endocardial recording and pacing in the remaining 22 mice as a simple atrial pacing group.After the carbachol(50 ng/g)was injected into the retro-orbital vein of mouse,the same combination of bursts was applied to the combined induction group mice.ECG electrophysiological parameters and AF/AFL duration were analyzed.Statisticalanalysis of data using the statistical product and service solutions 22.0 software.Results Spontaneous supraventricular arrhythmias were not observed in the sham-operated group(n=20);In the twice simple atrial pacing group(n=20),AF/AFL was induced in 4/20(20.0%)mice;In the simple pacing group,AF/AFL was successfully induced in 4/22(18.2%)mice with an average duration of(10.7±13.3)s.There was no statistically significant difference between the twice simple atrial pacing group and the simple pacing group(P>0.05);In the combined induction group,AF/AFL was induced in 13/20(65.0%)mice with an average duration of(44.6±171.7)s.There was a statistically significant difference between the simple pacing group and the combined induction group in the success rate of introduction and AF/AFL mean duration(P<0.05).Conclusion A simple and effective mouse model of AF/AFL can be established by rapid atrial pacing combined with carbachol.