葡萄籽油对甲巯咪唑诱导甲状腺功能减退小鼠的影响

Effect of Grape Seed Oil on Hypothyroid Induced by Methimazole of Mice

目的研究葡萄籽油对甲状腺功能减退小鼠的保护作用,并检测其对血清炎症因子白细胞介素(IL)-1β、IL-2和干扰素(INF)-γ表达的影响。方法 24只雌性小鼠随机分为正常对照组、模型对照组和葡萄籽油组,每组8只。模型对照组和葡萄籽油组小鼠采用甲巯基咪唑灌胃复制甲状腺功能减退动物模型。葡萄籽油组给予葡萄籽油1 mg·kg^(-1)·d^(-1)灌胃治疗4周。分离脾脏、胸腺和甲状腺组织,取血清检测小鼠甲状腺功能和细胞因子的含量。结果正常对照组、模型对照组、葡萄籽油组小鼠体质量分别为(23.54±1.06),(20.80±1.32),(20.28±0.82) g。葡萄籽油干预后小鼠脾质量较模型对照组显著降低(P<0.05)。经葡萄籽油干预后,血清游离三碘甲状腺原氨酸(FT_3)和游离甲状腺素(FT_4)的表达水平升高(P<0.05),葡萄籽油干预4周后,小鼠血清IL-1β和IL-2的表达水平显著降低(P<0.05)。结论葡萄籽油可能通过调节细胞因子IL-1β和IL-2的表达水平来减轻炎症反应从而达到改善甲状腺功能减退的作用。

Objective To study the protective effect of grape seed oil (GSO) on methimazole (MMI) induced hypothyroidism mice, and to test its effect on the expression of serum inflammatory factors IL-1 β,IL-2 and INF-γ. Methods A total of24 female mice were randomly divided into three groups: normal control group, model control group and grape seed oil group ( n =8). Animal model was constructed by gavage administration of MMI. Then the mice were sacrificed after1 mg·kg^-1 ·d^-1 GSO treatment for4 weeks. The spleen, thymus and thyroid tissue were separated . The function of thyroid gland and the content of cytokines in mice were mesured. Results Body weight of normal control group was (23.54±1.06) g, model control group was (20.80±1.32) g and grape seed oil group was (20.28±0.82)g. GSO could reduce the spleen quality comparing with model control group( P <0.05). GSO treatment could increase the production of FT3 and FT4 ( P <0.05) and significantly down-regulate the expression of IL-1β and IL-2 in serum. Conclusion GSO administration could provide protective effects on hypothyroidism through reducing inflammation and regulating the expression of IL-1β and IL-2.