苦瓜白粉病的生物防治及病程代谢物质含量变化

Studies on Biological Control and Changes of Metabolic Substances Content in Pathogenic Process of Bitter Gourd Powdery Mildew

利用枯草芽孢杆菌BLG010对苦瓜白粉病进行防治试验,同时为了探明苦瓜白粉病的病程相关物质,利用气相色谱-质谱联用技术分析白粉病菌接种处理、白粉病菌+BLG010接种处理和蒸馏水喷施处理后8 d和15 d时苦瓜叶片的代谢物质差异。试验结果表明,BLG010对苦瓜白粉病的防治效果高达89.49%;苦瓜感染白粉病菌后,D-葡萄糖、延胡索酸和肌醇半乳糖苷含量在8 d和15 d均上调表达,而甘氨酸、甘油酸、L-谷氨酸和咖啡酸则表现为下调。这7种代谢物质的含量变化与苦瓜白粉病病程相关,施用BLG010可以降低苦瓜白粉病病情指数,避免这7种代谢物质含量的异常变化。

This paper conducted experiment on controlling bitter gourd powdery mildew using Bacillus subtilis(BLG010).And in order to find out the pathogenic process-related substances,the paper analyzed the differences in metabolic substances of bitter gourd leaves 8 days and 15 days after pathogen inoculation treatment,pathogen+BLG010 inoculation treatment and distilled water spraying treatment by GC-MS methodology.The results showed that the control effect of BLG010 on bitter gourd powdery mildew was as high as 89.49%.After pathogen infection,the contents of D-glucose,fumaric acid and galactinol on 8 days and 15 days were all up-regulated.While,the contents of glycine,glyceric acid,L-glutamic acid and caffeic acid were down-regulated.Contents of these 7 metabolic substances were related to the pathogenic process of bitter gourd powdery mildew.Application of BLG010 can decrease the disease index,and avoid the abnormal changes in contents of these 7 metabolic substances.