二烯丙基一硫拮抗苯诱导小鼠白细胞降低效果研究

The protective effects of diallyl sulfide (DAS) on benzene-induced leukopenia in mice

目的研究二烯丙基一硫(DAS)拮抗苯诱导小鼠白细胞降低的效果。方法健康SPF雄性ICR小鼠90只,适应性饲养5 d后,随机分为空白对照组、苯模型组(1.30 g/kg)、苯+DAS低剂量组、苯+DAS中剂量组、苯+DAS高剂量组、DAS阳性对照组,每组15只。分别给予苯+DAS低、中、高剂量干预组和DAS阳性对照组小鼠40、80、160、160 mg/kg DAS,其余两组给予等体积的玉米油。2 h后,苯模型组和各干预组动物均经口给予苯-玉米油混悬液,其余两组给予等体积玉米油。苯和DAS溶解于玉米油中。1次/d,连续4周。分别于第14、29天麻醉后取血,收集抗凝血检测血常规,处死后,取小鼠脾脏和胸腺,用于计算脏器系数及组织病理学检查。结果与空白对照组比较,苯模型组小鼠第14天外周血白细胞、淋巴细胞、单核细胞计数分别降低68.99%、71.72%、53.19%,差异均有统计学意义(P<0.05);与染毒第14天比较,苯模型组小鼠第29天淋巴细胞、单核细胞、中性粒细胞、红细胞、血红蛋白分别降低83.00%、81.03%、89.37%、20.84%、19.25%,差异均有统计学意义(P<0.05);与空白对照组比较,苯模型组脾脏重量、脾脏系数、脾脏白髓面积比、胸腺重量、胸腺系数、胸腺皮质面积比均明显降低,差异均有统计学意义(P<0.05)。与苯模型组比较,第14天苯+DAS高剂量组单核细胞、淋巴细胞分别增加136.36%、260.00%(P<0.05);与干预第14天比较,第29天苯+DAS低、中、高剂量组白细胞、淋巴细胞、红细胞、血小板、血红蛋白均明显增高,差异均有统计学意义(P<0.05);与苯模型组比较,苯+DAS高剂量组脾脏重量、脾脏系数、脾脏白髓面积比、胸腺重量、胸腺系数、胸腺皮质面积比均明显升高,差异均有统计学意义(P<0.05)。结论DAS能有效拮抗苯诱导小鼠白细胞的降低。

Objective To study the protective effects of diallyl sulfide (DAS) on leukopenia induced by benzene. Methods 90 Healthy male ICR mice, adaptive feeding 5 days later, 15 were randomly divided into blank control group、model group、low、middle、high dose DAS intervention groups and DAS control group. Mice in intervention groups and DAS control group were orally given DAS at 40, 80, 160, 160 mg/kg·bw, while mice in the other two groups received an equal volume of corn oil. After 2 hours, model group and the other three intervention groups were given benzene, corn oil suspension (1.3 g/kg·bw), the two control groups treated with the same volume of corn oil, Benzene and DAS are dissolved in corn oil. one time for each day. 4 weeks later, Anesthesia at 14/29, make blood routine examination and count organ index and observe pathological examinations of spleen and thymus. Results On day 14, the counts of peripheral blood white blood cells (WBC), lymphocytes, monocytes in the model group decreased to 68.99%, 71.72%, 53.19%(P<0.05);On day 29, the counts of peripheral blood lymphocytes, monocytes, neutrophils in the model group decreased to 83.00%, 81.03%, 89.37%, 20.84%, 19.25%(P<0.05);spleen weight, spleen index, white pulp area ratio of spleen, thymus weight, thymus index, thymic cortex area ratio of mice in the model group decreased (P<0.05). On day 14, the counts of peripheral blood monocytes and lymphocytes in the DAS high dose intervention group increased by 136.36%, 260.00%(P<0.05);On day 29, the counts of White blood cells, lymphocytes, red blood cells, platelets, hemoglobin in the DAS low, middle and high dose intervention groups increased (P<0.05);spleen weight, spleen index, white pulp area ratio of spleen, thymus weight, thymus index, thymic cortex area ratio of mice in the DAS high dose intervention groups increased (P<0.05). Conclusion DAS can effectively suppress benzene-induced leucopenia in mice.