HIV感染者线粒体单倍群与神经认知障碍的关联研究

Association between mitochondrial haplogroups and neurocognitive disorder in HIV positive individuals

目的探索HIV感染者线粒体单倍群的分布,并探讨其与神经认知障碍(NCD)的关联。方法利用2017年1-12月浙江省台州市“HIV与衰老相关疾病前瞻性队列研究”基线数据,采用Sanger测序法对HIV感染者的线粒体基因进行全基因组测序和分型,使用简易精神状态量表(MMSE)对浙江省台州市448例HIV感染者进行问卷调查测量其NCD患病率,通过多因素logistic回归分析检验探索线粒体单倍群与NCD的关联。结果448例HIV感染者中,线粒体单倍群D(19.6%,88/448)、B(19.4%,87/448)和F(17.0%,76/448)人数最多。NCD患病率为20.3%(91/448),单倍群A、D、F和M7的HIV感染者NCD患病率较高,分别为23.1%(9/39)、21.6%(19/88)、26.3%(20/76)和26.1%(12/46)。调整年龄、性别等混杂因素后,多因素logistic回归分析结果显示,与线粒体单倍群A的HIV感染者相比,单倍群B、D、F、M7、M8、N9及其他单倍群HIV感染者的NCD患病率差异无统计学意义。结论本研究初步探索了HIV感染者中线粒体单倍群与NCD之间的关联性,未发现线粒体单倍群与NCD存在关联,未来需要大样本前瞻性队列研究。

Objective To investigate the distribution of mitochondrial haplogroups and their correlation with neurocognitive disorder (NCD) in HIV positive individuals. Methods Baseline data were from the prospective cohort study of comparative HIV and aging research in Taizhou of Zhejiang province from January to December, 2017. A cross-sectional survey was performed in 448 HIV positive individuals. Sanger method was used for the sequencing and genotyping of whole mitochondrial genome of HIV positive individuals. NCD prevalence in the HIV positive individuals was assessed by Mini-mental State Examination (MMSE) in questionnaire interviews. Multivariable logistic regression analysis was performed to assess the associations between mtDNA haplogroups and NCD. Results In this sample, mitochondrial haplogroups D (19.6%, 88/448), B (19.4%, 87/448) and F(17.0%, 76/448) were the most predominant haplogroups. The overall prevalence rate of NCD was 20.3%(91/448), and was high in haplogroups A (23.1%, 9/39), D (21.6%, 19/88), F (26.3%, 20/76) and M7 groups (26.1%, 12/46), respectively. In multivariable logistic regression analysis after adjusting confounding factors, such as age and gender, compared with haplogroup A, there were no differences in the prevalence rate of NCD among HIV positive individuals with haplogroup B, D, F, M7, M8, N9, and others. Conclusion The study explored primarily correlation between mitochondrial haplogroups and NCD among HIV positive individuals and suggested that there is no significant association between mitochondrial haplogroups and NCD, but further longitudinal investigation with large sample size of HIV positive population is needed to confirm this finding.