射频消融治疗对肝癌患者细胞免疫功能的影响

Effect of radiofrequency ablation on cellular immune function in patients with liver cancer

目的探讨射频消融治疗对肝癌患者细胞免疫功能的影响。方法对60例肝癌患者应用射频消融术治疗,于治疗前24h、治疗1周及4周末采用流式细胞仪检测患者外周血T淋巴细胞亚群及CD4^+CD25^+调节性T细胞的比例变化,治疗4周末依据临床症状缓解情况评定临床疗效。结果治疗4周末,本组患者治疗总有效率为50%。射频消融治疗1周、4周末患者外周血中CD3^+、CD4^+及CD4^+/CD8^+水平均较治疗前升高,CD4^+CD25^+调节性T细胞较治疗前降低,差异有统计学意义(P<0.05);CD8^+水平略升高,差异无统计学意义(P>0.05)。与治疗1周末比较,治疗4周末CD3^+、CD4^+及CD4^+/CD8^+比例略有升高,CD4^+CD25^+调节性T细胞比例略升高,差异无统计学意义(P>0.05)。结论原发性肝癌患者存在细胞免疫抑制,射频消融治疗能明显提高患者的细胞免疫功能。

Objective To investigate the effect of radiofrequency ablation on cellular immune function in patients with liver cancer.Methods 60 patients with liver cancer were treated by radiofrequency ablation.Flow cytometry was used to detect the proportion of peripheral blood T lymphocyte subsets and CD4^+CD25^+ regulatory T cells at 24 h before treatment,1 week and 4 weeks after treatment.At the end of the 4th week of treatment,the clinical efficacy was evaluated according to the clinical symptom relief.Results At the end of the 4th week of treatment.the total effective rate of treatment in this group of patients was 50%.At the end of the first,4th week of treatment,the levels of CD3^+,CD4^+ and CD4^+/CD8^+ in peripheral blood of patients were higher than those before treatment,and CD4^+CD25^+ regulatory T cells were lower than before treatment,the difference was statistically significant(P<0.05).The level of CD8^+ was slightly elevated,and the difference was not statistically significant(P>0.05).Compared with the first week of treatment,the proportion of CD3^+ CD4^+ and CD4^+/CD8^+ increased slightly at the end of the 4th week,and the proportion of CD4^+CD25^+ regulatory T cells increased slightly,the difference was not statistically significant(P>0.05).Conclusion Cellular immunosuppression exists in patients with primary liver cancer,and radiofrequency ablation can significantly improve the cellular immune function of patients.