三种不同止血方法在大鼠肛管直肠出血模型中的止血效果

Hemostatic effect of three different hemostasis methods in the model of anorectal hemorrhage in rats

目的评估三种不同止血方法在大鼠肛管直肠出血模型中的止血效果。方法 27只SPF级SD雄鼠随机分为气囊腔道止血器组(A组)和明胶海绵组(B组)、凡士林油纱组(C组),每组9只。建立出血模型,分别用气囊腔道止血器压迫,凡士林油纱或明胶海绵填塞止血,首次施压4 min,然后每隔2 min检查出血至止血,观察止血时间、计算失血量。止血成功后,观察5 min评估有无再出血。术后2 d取创面苏木精-伊红染色显微镜观察炎症情况。结果与C组、B组比较,A组止血时间更短、失血量更少,差异有高度统计学意义(P<0.01);与B组比较,C组失血量较少,差异有统计学意义(P<0.05);三种止血方法 5 min内再出血率比较,差异无统计学意义(P>0.05)。组织切片显示C组炎性反应最严重。结论最有效的止血方法是气囊腔道止血器,其次是凡士林油纱,明胶海绵单独使用止血效果差。

Objective To evaluate the hemostatic effect of three different hemostatic methods in the model of anorectal bleeding in rats. Methods Twenty-seven SPF SD male rats only randomized block design was divided into air tract tourniquet group (group A) and gelatin sponge group (group B), vaseline oil gauze group (group C), each group of nine, hemorrhage model was established, with balloon tourniquet pressure cavity, vaseline oil gauze or gelatin sponge tamponade hemostasis, press 4 min, for the first time and then every 2 min to check bleeding to stop bleeding, observe the bleeding time, blood loss, after the success of the hemostatic, observe 5 min to assess any bleeding again. Two days after surgery, hematoxylin - eosin staining was used to observe the inflammation. Results Compared with group C and B, group A had shorter hemostasis time and less blood loss, and the difference was statistically significant (P<0.01). Compared with group B, the blood loss in group C was lower, and the difference was statistically significant (P<0.05).There was no statistically significant difference between the three hemostasis methods for re-bleeding within 5 min (P > 0.05). Histological sections showed the most severe inflammatory response in group C. Conclusion The most effective hemostasis method is the balloon cavity hemostatic device, followed by vaseline gauze, gelatin sponge used alone has poor hemostatic effect.