在线固相萃取-高效液相色谱-串联质谱法测定饮用水中4种兴奋剂

Determination of 4 Stimulants in Drinking Water ― On-line Solid Phase Extraction Followed by High Performance Liquid Chromatography-Tandem Mass Spectrometry

可替宁、可待因、1,7-二甲基黄嘌呤和咖啡因作为药物及其代谢产物广泛应用于日常生活中,其在环境中的残留物可能对人类健康和生态环境安全造成威胁。采用在线固相萃取法进行前处理,酸性条件(0.1%甲酸水溶液)上样,水样加硫代硫酸钠(1L水样加0.1g硫代硫酸钠)除去余氯后用高效液相色谱-串联质谱法测定饮用水中可替宁、可待因、1,7-二甲基黄嘌呤和咖啡因这4种兴奋剂。当进样体积为1.0mL时,方法检出限为0.633~1.75ng/L,测定下限为2.53~6.98ng/L,饮用水样品加标回收率为80.0%~129%,多次测定均值的相对标准偏差(RSD)<5%。方法检出限低,精密度和准确度好,能满足饮用水样品中4种兴奋剂的日常检测。

Cotinine,Codeine,1,7-Dimethylxanthine and Caffeine are widely used in daily life as drugs and their metabolites.The stimulant residues in the environment may pose a threat to human health and ecological environment safety.Water samples were sampled under acidic conditions (0.1% formic acid aqueous solution) and the residual chlorine was removed by adding sodium thiosulfate (1 L water sample with 0.1 g sodium thiosulfate) to the water samples.On-line solid phase extraction followed by LC-MS-MS was used to determine 4 stimulants in drinking water,including Cotinine,Codeine,1,7-Dimethylxanthine and Caffeine.When the injection volume was 1.0 mL,the detection limit of this method was 0.633~1.75 ng/L,the low limit of determination was 2.53~6.98 ng/L and the recoveries of standard-added drinking water samples ranged from 80.0% to 129%.The method has low detection limit and high recovery,and can meet the requirements of the daily detection of four stimulants in drinking water samples.