二烯丙基二硫诱导人胃癌MGC803细胞的差异microRNA表达

The differential expression of microRNA in human gastric cancer MGC803 cells induced by diallyl disulfide

目的研究二烯丙基二硫(diallyl disulfide,DADS)诱导人胃癌MGC803细胞的差异miRNAs表达。方法采用miRNAs芯片与qRT-PCR检测DADS诱导人胃癌MGC803细胞的差异miRNAs表达。结果 microRNA芯片检测显示,30 mg·L^(-1)DADS处理24 h后,MGC803细胞的差异miRNAs表达中,miR-200b、miR-22、miR-7、miR-143、miR-138、miR-34a、miR-150等7个miRNA表达明显上调,miR-222、miR-21、miR-15b、miR-182、miR-18a等5个miRNA下调。q PCR证实,30 mg·L^(-1)DADS处理MGC803细胞后,miR-200b、miR-22、miR-7、miR-143、miR-138、miR-34a、miR-150等表达较未处理组明显上调(P <0. 05)。且q PCR检测显示,miR-200b与miR-22在MGC-803、BGC-823、MKN-28、SGC-7901、HGC-27等各种人胃癌细胞表达较正常人胃癌GES-1细胞明显下调(P <0. 05)。miR-200b与miR-22在胃癌组织中表达较正常胃组织明显下调(P <0. 05)。结论DADS诱导人胃癌MGC803细胞的差异miRNAs表达有7个miRNA明显上调,5个miRNA下调。miR-200b与miR-22在胃癌组织与细胞中表达下调。DADS可上调MGC803细胞miR-200b与miR-22表达。

Aim To investigate the expression and identification of differential miRNAs in human gastric cancer MGC803 cells induced by diallyl disulfide(DADS). Methods Differential miRNAs expression in human gastric cancer MGC803 cells induced by DADS was detected and identified by miRNA chip and qPCR. Results MiRNAs chip detection showed up-regulation of miR-200b, miR-22, miR-7, miR-143, miR-138, miR-34a and miR-150, and down-regulation of miR-222, miR-21, miR-15b, miR-182 and miR-18a in differential miRNAs of MGC803 cells treated with 30 mg·L^-1 DADS at 24 h( P <0.05). And qPCR demonstrated that the expressions of miR-200b, miR-22, miR-7, miR-143, miR-138, miR-34a and miR-150 was up-regulated in MGC803 cells treated with 30 mg·L ^-1 DADS( P <0.05). Moreover, qPCR showed that the expressions of miR-200b and miR-22 in various human gastric cancer cells including MGC803, BGC823, MKN28, SGC7901 and HGC27 cells were lower than normal human gastric cancer GES-1 cells( P <0.05). The expression of miR-200b and miR-22 in gastric cancer tissues was significantly lower than that in normal gastric tissues( P <0.05). Conclusions The expression of down-regulation of 7 miRNA and up-regulation of 5 miRNA in differential miRNAs in MGC803 cells induced by DADS. The expression of down-regulation of miR-200b and miR-22 in gastric cancer tissues and cells. DADS could up-regulate the expression of miR-200b and miR-22 in gastric cancer cells.