去势比格犬腹部脂肪与相关血清学指标及骨密度的变化关系

The relationship between abdominal fat tissue, bone mineral density and serum biochemistry parameters in ovariectomized beagle dogs

目的通过建立去势比格犬模型,观察绝经早期腹部脂肪变化规律,并通过对脂肪与骨代谢相关血清学指标的测量与分析,探讨脂肪及骨代谢的关键影响因素。方法选取6只成年雌性比格犬进行去势术,分别在术前、术后4个月、6个月、10个月进行腰椎定量CT(quantatitive computed tomography,QCT)腹部脂肪面积、骨密度(bone mineral density,BMD)、MRI腰椎骨髓脂肪含量及血清学指标的检测,比较不同时间各指标的变化趋势及关系。结果比格犬腹内脂肪面积(visceral fat area,VFA)、皮下脂肪面积(subcutaneous fat area,SFA)、腹部总脂肪面积(total fat area,TFA)在术后6个月、10个月均增加(P<0.05),术后10个月VFA增加百分比均值为84.39%,且为三者中最大;术后比格犬BMD并未明显降低。体重、BMD、瘦素(leptin,LP)、VFA、高密度脂蛋白(high-density lipoprotein,HDL)与SFA相关。SFA、体重、低密度脂蛋白(low-density lipoprotein,LDL)、内脏脂肪素(visfatin,VFN)与BMD相关。结论去势比格犬模型可用于研究绝经后雌激素缺乏所引起的脂肪代谢变化,但短期内BMD并未明显丢失,骨、脂肪代谢之间存在交互作用。

Objective To observe the changes of abdominal fat tissue in ovariectomized beagle dogs, and to explore the key factors that influence the metabolism of lipid and bone through the measurement and analysis of serum indexes. Methods Six mature female beagle dogs were ovariectomized. Quantitative computed tomography(QCT) and MR mDixon-quant examinations, respectively, were used to determine the abdominal fat area, BMD of the lumbar vertebrae, and the percentage of bone marrow fat content on pre-surgery, 4, 6, and 10 months post-surgery. Serum biochemical indexes were also measured. The relationship and change trend at different times were compared. Results The VFA, SFA, TFA increased in 6 and 10 months post-surgery(P<0.05). The average growth rate of VFA in10-month post-surgery was 84.39%, and it was the highest among the three indexes. The post-surgery BMD did not decrease. Weight, BMD, LP, VFA, HDL were correlated with SFA. SFA, weight, LDL, and VFN were correlated with BMD. Conclusion This model can be used to simulate and study fat distribution in menopausal women. However, BMD does not decline in a short term after operation. There is interaction between the metabolism of lipid and bone.