Fractalkine在大鼠肝硬化中全肝血流阻断致肺损伤中表达

Expression of Fractalkine in lung injury induced by total hepatic blood flow occlusion in rat liver cirrhosis model

目的探讨趋化因子Fractalkine(Fkn,CX3CL1)在大鼠肝硬化模型中全肝血流阻断致肺损伤中表达及意义。方法选择80只雄性成年SD大鼠,鼠龄2.0～2.5个月,体质量160～200 g。随机分为4组(n=20),即对照组(A组),阻断肝门、肝上下腔静脉及肝下下腔静脉15 min(B组)、30min(C组)、45 min(D组);制作肝硬化大鼠全肝血流阻断模型。取右肺下叶肺组织常规苏木精-伊红(HE)染色病理检查,留取肺及肝组织检测Fkn的表达,并切取肺组织及血浆检测核因子-κB(NF-κB)及白细胞介素21(IL-21)水平变化。结果各组成功建模后,D组肺损伤病理评分[(12.25±1.52)分]明显高于A组[(2.05±1.00)分]、B组[(7.90±1.62)分]及C组[(8.25±1.55)分](P<0.001),A组无明显病理改变。在各组肝、肺组织中Fkn的表达测定中,D组Fkn表达明显高于B组、C组及A组(P<0.05)。血清中NF-κB、IL-21表达在A组[(11.67±2.11) ng/L,(633.30±42.03) ng/L]最低(P<0.05),D组[(18.13±1.83) ng/L,(801.96±54.38) ng/L]明显高于B组[(14.72±2.56) ng/L,(712.67±53.63) ng/L]及C组[(15.23±2.26) ng/L,(713.59±61.60) ng/L](P<0.05)。肺组织中NF-κB、IL-21表达A组[(14.48±2.07) ng/L,(590.89±55.38) ng/L]最低(P<0.05),D组[(19.66±1.53) ng/L,(910.85±87.63) ng/L]明显高于B组[(16.65±1.75) ng/L,(696.23±51.91) ng/L]及C组[(17.02±1.47) ng/L,(697.22±41.24)ng/L](P<0.05)。结论 Fkn在肝硬化大鼠全肝血流阻断致肺组织损伤时表达增加,且阻断时间超过30 min后更加明显。

Objective To investigate the expression and significance of Fractalkine(Fkn) in lung injury induced by total hepatic blood flow occlusion in rat liver cirrhosis model. Methods Eighty adult male SD rats aged 2.0-2.5 months old with body weight of 160-200 g were randomly divided into 4 groups(n = 20): control group(group A, hilar, supra-supercavity, and inferior liver were blocked), group B(the hepatic cirrhosis rats were subjected to hepatic hepatic occlusion for 15-minute), group C(30-minute) and group D(45-minute). The pathological examination of right lower lobe lung tissue was performed by hematoxylineosin(HE) staining, lung and liver tissues were collected for detection of Fkn expression, and lung tissue and plasma were harvested for detection of nuclear factor kappa-B(NF-KB) and interleukin-21(IL-21) levels. Results After successful modeling,the pathological scores of lung injury in group D[(12.25± 1.52) scores] were significantly higher than those in group A[(2.05 ±1.00) scores], group B[(7.90 ± 1.62) scores] and group C[(8.25 ± 1.55) scores](P < 0.001), while there was no significant pathological changes in group A. The expression of Fkn in group D was significantly higher than that in group B, group C and group A(P< 0.05). The expression of serum NF-κB and IL-21 was the lowest in group A(11.67 ng/L ± 2.11 ng/L, 633.30 ng/L ±42.03 ng/L)(P< 0.05),and expression of NF-κB and IL-21 in group D(18.13 ng/L ± 1.83 ng/L, 801.96 ng/L ± 54.38 ng/L) was higher than that in group B(14.72 ng/L ± 2.56 ng/L, 712.67 ng/L ± 53.63 ng/L) and group C(15.23 ng/L ± 2.26 ng/L, 713.59 ng/L ±61.60 ng/L)(P< 0.05). The expression of NF-κB and IL-21 in lung tissue was the lowest in group A( 14.48 ng/L ± 2.07 ng/L590.89 ng/L ± 55.38 ng/L)(P< 0.05),and expression of NF-κB and IL-21 in group D(19.66 ng/L ± 1.53 ng/L, 910.85 ng/L ±87.63 ng/L) was significantly higher than that in group B(16.65 ng/L ± 1.75 ng/L,696.23 ng/L ± 51.91 ng/L) and group C(17.02 ng/L ± 1.47 ng/L, 697.22 ng/L ± 41.24 ng/L)(P < 0.05). Conclusion It is demonstrated that the expression of Fkn is increased in lung tissue injury induced by total hepatic blood flow occlusion in cirrhotic rats, and it is more obvious after the blockage time exceeds 30-minute.