白刺花中3种生物碱的含量及HPLC指纹图谱

HPLC fingerprint and contents of three kinds of alkaloids of Sophora davidii (Franch.) Skeels

为建立白刺花高效液相指纹图谱,并测定白刺花不同部位(根、茎、叶)苦参碱、槐果碱和氧化苦参碱的含量。实验采用AgilentTC-C_(18)(2)色谱柱(250×4.6 mm,5μm),以乙腈-0.09%三乙胺水溶液(4∶96,磷酸调pH3.5)为流动相,流速为1.0 mL/min,紫外检测波长205 nm,柱温35℃。建立了白刺花的高效液相指纹图谱,确定了14个共有峰,样品的相似度在0.9以上。在该色谱条件下,各成分有较好的分离度,苦参碱、槐果碱和氧化苦参碱分别在6.34～101.5、6.47～103.5、8.38～100.5μg/mL范围内呈良好的线性关系(r>0.999)。用该方法测得白刺花根中苦参碱、槐果碱、氧化苦参碱的平均含量分别为8.204 8、1.237 1、66.147 6 mg/g;茎中分别为4.246 3、1.549 6、25.035 1 mg/g;叶中分别为9.314 8、6.947 4、1.573 4 mg/g。该方法简单、准确,可用于白刺花的质量控制。

To establish HPLC fingerprint and determine the contents of three kinds of alkaloids in Sophora davidii ( Franch.) Skeels. The chromatographic analysis were carried out on a AgilentTC-C18 column ( 250 mm × 4. 6 mm,5 μm) with acetoni- trilel-0. 09% triethylamine ( phosphoric acid adjusted to pH3. 5) for the split 4∶ 96 as the mobile phase at a flow rate of 1. 0 mL/min. The detect wavelength was set at 205 nm,and the column temperature was set at 35 ℃. The HPLC fingerprint of S. Davidii was established,and 14 common peaks were determined. The similarity of the sample was above 0. 9. Under these chromatographic conditions,Under the chromatographic conditions,each component has a good resolution,three alkaloids( ma- trine,sophocarpine and oxymatrine) have a good linear relationship in the range of 6. 34-101. 5,6. 47-103. 5 and 8. 38-100. 5 μg /mL,respectively. The average content of three alkaloids in the roots was 8. 204 8,1. 237 1 and 66. 147 6mg /g,respective- ly. Simultaneously,the content in the stems was 4. 246 3,1. 549 6,25. 035 1 mg /g,and in the leaves it was 9. 314 8,6. 947 4,1. 573 4 mg /g. The method is simple and accurate,and can be used for quality control of S. Davidii.