红豆树叶挥发油化学成分及其抗氧化和抑菌活性研究

Chemical constituents of the volatile oil from Ormosia hosiei leaves and its antioxidant and antimicrobial activity

为探究红豆树叶挥发油的化学组成及其生物活性,本研究首次采用水蒸气蒸馏法提取红豆树叶挥发油,并通过气相色谱-质谱联用技术(GC-MS)分析其化学成分,结合DPPH和ABTS法及抑菌圈法,评价其体外抗氧化和抑菌活性。结果表明,从红豆树叶挥发油中共检测出化合物36个,占挥发油总量的90.50%;挥发油主要成分为1,4-二十烷二烯(25.72%)、1,19-二十烷二烯(10.85%)、2,6-二叔丁基对甲酚(10.14%)、邻苯二甲酸正丁基异丁基酯(9.75%)、(Z,Z)-6,9-二十烷二烯(7.60%)、(E,E)-α-金合欢烯(7.51%)、叶醇(4.74%)和2-异丙烯基-5-甲基-6-庚烯-1-醇(4.04%)。红豆树叶挥发油对DPPH自由基和ABTS自由基清除作用的半数有效量(ED_(50))分别为0.27、0.14 mg/mL,且抗氧化活性与挥发油浓度呈量效相关。红豆树叶挥发油浓度为7.1 mg/mL时,其对枯草芽孢杆菌(Bacillus subtilis)、金黄色葡萄球菌(Staphylococcus aureus)、绿脓杆菌(Pseudomonas aeruginosa)和大肠杆菌(Escherichia coli)的抑菌圈分别为11.29、9.88、10.85和11.03 mm。本研究为红豆树叶资源的综合开发利用提供了理论基础。

In this study,we firstly reported that the volatile oil from Ormosia hosiei Hemsl. Et Wils. leaves was extracted by water-steam distillation and then analyzed by gas chromatography-mass spectrometry ( GC-MS). The antioxidant activity of the volatile oil was determined by free radical scavenging assays against 1,1-diphenyl-2-picrylhydrazine ( DPPH) and 2,2'-azino- bis-( 3-ethylbenzothiazoline-6-sulphonic acid)( ABTS). And its antimicrobial activity was also examined by inhibition zone. The results showed that thirty-six compounds,which represented 90. 50% of the total constituents,were identified from the volatile oil. The major compounds of the volatile oil were 1,4-eicosadiene ( 25. 72%),1,19-eicosadiene ( 10. 85%),2,6-di- tert-butyl-4-methylphenol ( 10. 14%),butyl isobutyl phthalate ( 9. 75%),( Z,Z)-6,9-eicosadiene ( 7. 60%),( E,E)-α-far- nesene ( 7. 51%),leaf alcohol ( 4. 74%) and 2-isopropenyl-5-methyl-6-hepten-1-ol ( 4. 04%). The volatile oil exhibited sig- nificant antioxidant activities against DPPH and ABTS free radicals in a concentration-dependent fashion,with the median ef- fective dose ED50 values of the antioxidant activities against DPPH and ABTS free radicals were 0. 27 mg /mL and 0. 14 mg / mL,respectively. When the concentration of the volatile oil was 7. 1 mg /mL,the inhibition zone of Bacillus subtilis,Staphylo- coccus aureus,Escherichia coli,and Pseudomonas aeruginosa was 11. 29,9. 88,10. 85,and 11. 03 mm,respectively. These re- sults provide theoretical basis for the comprehensive development and utilization of the natural resources of O. hosiei Hemsl. Et Wils.