16种茯苓菌株液体发酵产胞内多糖与三萜的比较研究

Comparative study on the intracellular polysaccharides and triterpenoids produced by 16 Wolfiporia cocos strains with submerged fermentation strategies

筛选茯苓高产胞内多糖和胞内三萜的优良液体发酵出发菌株。采用PDA富集固体平板培养与液体发酵培养测定菌丝体生长速率;采用液体发酵策略分析16种茯苓菌株产胞内多糖与胞内三萜的潜能。实验结果表明菌株生长于固体培养基与种子培养基的生长速率之间没有关联性;降低一级种子培养基初始pH值到4.0时能有效缓解茯苓菌株培养物褐化现象;AS5.137胞内多糖含量最高,达377.60±0.10 mg/g,而DB菌株显示出最高的胞内多糖产量,达1.01±0.13 g/L;Y1菌株胞内三萜含量最高,达83.89±4.28 mg/g,而Jingzhou28菌株胞内三萜产量最高,达136.63±26.66 mg/L。就生产茯苓胞内多糖与胞内三萜而言,AS5.137与DB菌株适合作为液体发酵产胞内多糖的出发菌株;Y1,Jingzhou28,Z(z)与Xingpinzhong菌株均较适合作为液体发酵产胞内三萜的出发菌株。

Experiments were performed aimed to screen out submerged fermentation start Wolfiporia cocos strains,which should be high intracellular polysaccharide yield and triterpenoid yield.PDA enrichment plates and submerged fermentation cultures were adopted to determine the mycelium growth rate,respectively.The submerged fermentation strategy was employed to analyze the potentials of 16 W.cocos strains producing intracellular polysaccharides and intracellular triterpenoids.The data from mycelium growth rate determination assays demonstrated that there is no correlation between the growth rate of mycelium grown on PDA plate and that cultured in seed medium.And our data showed that the browning phenomenon of W.cocos could be alleviated by decreasing the initial pH value of primary seed medium to 4.0.Furthermore,AS5.137 exhibited the highest intracellular polysaccharide content,up to 377.60±0.10 mg/g,but DB displayed the highest intracellular polysaccharide yield,up to 1.01±0.13 g/L.Y1 produced the most amount of intracellular triterpenoids,and the corresponding value was 83.89±4.28 mg/g.However,Jingzhou28 displayed the highest intracellular triterpenoids,up to 136.63±26.66 mg/L.As far as on the production of intracellular polysaccharides and triterpenoids of W.cocos is concerned,the data from this study suggested that AS5.137 and DB are suitably employed as submerged fermentation start strains for producing intracellular polysaccharides,while Y1,Jingzhou28,Z(z),and Xingpinzhong as submerged fermentation start strains for producing intracellular triterpenoids.