摘要
采用高效液相法对柴芍口服液指标性成分芍药苷含量进行测定。色谱柱:ZorbaxSBC18(4.6mm×250mm,5μm);流动相:乙腈+0.05%磷酸溶液(14∶86);流速:1.0mL/min;柱温:35℃;检测波长:230nm。经精密度、稳定性、重复性、回收率等验证,在该色谱条件下能够达到对柴芍口服液的质量控制。
The content of paeoniflorin in Chaishao Oral Liquid was determined by high performance liquid chromatography. The column was Zorbax SB C18(4.6 mm × 250 mm, 5 μm), the mobile phase was acetonitrile+0.05% phosphoric acid solution(14∶86), the flow rate was 1.0 mL/min, the column temperature was 35℃,and the detection wavelength was 230 nm.Under these conditions,the quality control of Chaishao Oral Liquid could be achieved by the verification of precision,stability,repeatability and rate of recovery.
作者
张会梅
孙亚磊
王艳玲
钟英杰
魏秀丽
付海宁
栾明娜
庞云露
蒋贻海
徐恩民
Zhang Huimei;Sun Yalei;Wang Yanling;Zhong Yingjie;Wei Xiuli;Fu Haining;Luan Mingna;Pang Yunlu;Jiang Yihai;Xu Enmin(Qingdao Vland Biotech INC Co.,Ltd.,Qingdao 266061,China;Qingdao National Engineering Technology Research Center of Animal Health Care Products Co.,Ltd.,Qingdao 266111,China;Shandong Veterinary Drug Quality Inspection Institute,Jinan 250022,China)
出处
《山东农业科学》
2019年第5期144-147,共4页
Shandong Agricultural Sciences
基金
国家“十三五”重点研发计划项目(2017YFD0501505)
科技惠民项目(17-3-3-43-nsh)
山东省重点研发计划项目(2018GNC111010)
关键词
柴芍口服液
高效液相色谱
芍药苷
Chaishao Oral Liquid
High performance liquid chromatography(HPLC)
Paeoniflorin
