摘要
根癌农杆菌介导转化法是植物基因工程的重要方法,但其转化效率受多种因子的影响,包括化学物质、物理方法、外植体遗传特性等,涉及农杆菌与植物互作的各个阶段。将这些影响因子及其功能进行简要综述,以期为优化农杆菌介导植物遗传转化流程提供帮助。
Agrobacteriumt umefaciens-mediated transformation is an important method of plant genetic engineering. Agrobacterium tumefaciens-mediated transformation efficiency is affected by various factors, including chemical substances, physical methods, genetic characteristics of the explants, and so on. They involve each stage of interaction between Agrobacterium and plant cells. In this paper, these factors and their functions are summarized to help researchers optimzing the process of by Agrobacterium-mediated plant genetic transformation.
作者
叶文兴
孔令琪
YE Wen-xing;KONG Ling-qi(College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019,China;Instutute of Grassland Research of Chinese Academy of Agriculture Sciences,Hohhot 010010,China)
出处
《中国草地学报》
CSCD
北大核心
2019年第3期142-148,共7页
Chinese Journal of Grassland
基金
国家自然科学基金“活性氧对羊草种子萌发的影响及其作用机理研究”(31702172)
内蒙古自治区自然科学基金“乙烯与油菜素内酯对盐胁迫下苜蓿种子萌发机制的研究”(2016BS0319)
关键词
根癌农杆菌
TI质粒
T-DNA整合
染色体
转化效率
Agrobacterium tumefaciens
Ti plasmid
T-DNA integration
Chromosome
Transformation efficiency
