加味阳和汤对肾阳虚型骨质疏松模型大鼠骨密度及血清微量元素的影响

Effect of Jiawei Yanghe Decoction on Bone Mineral Density and Serum Trace Elements in Osteoporosis Rats with Kidney Yang Deficiency

目的探究不同浓度的加味阳和汤对维甲酸灌胃、氢化可的松臀部肌肉注射造成的肾阳虚型骨质疏松症(osteoporosis,OP)模型大鼠骨密度(bone mineral density,BMD)及血清微量元素的影响。方法将48只SD大鼠分为空白对照组,模型组,加味阳和汤低、中、高剂量组,阳性对照组,除空白对照组外其余均复制肾阳虚型OP模型鼠,分别给予1%羧甲基纤维素钠、加味阳和汤(5、10、20 g/kg)、戊酸雌二醇(estradiol valerate,E2V)(0.000 2 g/kg)灌胃,每日1次,持续灌胃6周。取大鼠股骨及血清,观察大鼠股骨BMD、血清碱性磷酸酶(alkaline phosphatase,ALP)、抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphatase,TRAP)水平及微量元素[镁(magnesium,Mg)、锌(zinc,Zn)、铁(ferrum,Fe)]的变化。结果与模型组比较,加味阳和汤各剂量组大鼠BMD均有不同程度的上升,大鼠血清ALP、TRAP活性均显著降低( P< 0.05),血清Fe、Zn水平均显著升高,差异有统计学意义(P<0.05),血清Mg水平的变化无统计学意义(P>0.05)。结论加味阳和汤对肾阳虚型OP大鼠有明显的治疗作用,且其作用机制可能与调节血清微量元素水平,抑制骨的高转换率有关。

Objective To investigate the effect of different concentrations of Jiawei Yanghe Decoction on bone mineral density (BMD) and serum trace elements in a rat model of osteoporosis (OP) with kidney Yang deficiency induced by intragastric administration of retinoic acid and gluteal intramuscular injection of hydrocortisone. Methods A total of 48 Sprague-Dawley rats were divided into blank control group, model group, low-, middle-, and high-dose Jiawei Yanghe Decoction groups, and positive control group. All rats except those in the blank control group were used to establish a model of OP with kidney Yang deficiency. The rats in the model group, low-, middle-, and high-dose Jiawei Yanghe Decoction groups, and positive control group were given 1% sodium carboxymethyl cellulose, Jiawei Yanghe Decoction (5, 10, and 20 g/kg ), and estradiol valerate (0.0002 g/kg), respectively, by gavage, once a day for 6 consecutive weeks. The femur and serum were collected to measure BMD of the femur and serum levels of alkaline phosphatase (ALP), tartrate-resistant acid phosphatase (TRAP), and the trace elements magnesium (Mg), ferrum (Fe), and zinc (Zn). Results Compared with the model group, the low-, middle-, and high-dose Jiawei Yanghe Decoction groups had significant increases in BMD, significant reductions in serum ALP and TRAP, and significant increases in serum Fe and Zn (all P<0.05), while there was no significant difference in serum Mg level between the two groups (P>0.05). Conclusion Jiawei Yanghe Decoction exerts a marked therapeutic effect on OP rats with kidney Yang deficiency, possibly by regulating the serum levels of trace elements and inhibiting the high bone turnover rate.