清温并用法对慢加急性肝衰竭大鼠结肠组织调节性T淋巴细胞/辅助性T淋巴细胞17细胞因子的影响

Effects of Clearing and Warming Simultaneously on Colonic Regulatory T Lymphocytes/Assistant T Lymphocyte 17 Cytokines in Rats with Acute and Chronic Liver Failure

目的研究清温并用法对慢加急性肝衰竭(acute-on-chronic liver failure,ACLF)大鼠结肠组织Treg/Th17相关型细胞因子表达的影响,探讨其抗ACLF肠源性内毒素血症(intestinal endotoxemia,IETM)的可能调控机制。方法140只SD大鼠随机分为正常组、模型组、清法组、温法组及清温并用法组。牛血清白蛋白致敏法建立免疫性肝纤维化大鼠模型,腹腔注射D-氨基半乳糖+脂多糖急性攻击建立ACLF大鼠模型,各组予以相应干预,观察24 h内各组大鼠外周血内毒素、外周血Treg/Th17细胞比值、结肠组织Treg/Th17型细胞因子变化。结果与正常组比较,模型组大鼠在各时间点内毒素明显升高(P<0.01)、Treg/Th17型细胞因子明显升高(P<0.01)、Treg/Th17比值明显降低(P<0.01);与模型组比较,清法、温法、清温并用法组在1、12、24 h各个时间点内毒素、Treg/Th17相关细胞因子表达均降低(P<0.05),以清温并用法组下降最显著(分别与清法组、温法组比较,P<0.05);各组与模型组比较,仅清温并用法组Treg/Th17比值明显升高(P<0.05)。结论清温并用法抗肝衰竭IETM的机制可能与调节结肠组织Treg/Th17型细胞因子失衡,促使Treg/Th17细胞恢复平衡有关。

Objective To investigate the effects of clearing and warming simultaneously on the expression of Treg/Th17 associated cytokines in colon tissue of rats with acute and chronic liver failure(ACLF),and to explore its possible regulation mechanism of ACLF against intestinal endotoxemia(IETM).Methods A total of 140 SD rats were randomly divided into a normal group,a model group,a clearing group,a warming group,and a clearing and warming simultaneously group.The rats model of immune hepatic fibrosis were established by sensitization with bovine serum albumin,and then ACLF rats were induced by intraperitoneal injection of D-galactose and lipopolysaccharide(LPS).The changes of endotoxin in peripheral blood,ratio of Treg/Th17 cells in peripheral blood and Treg/Th17 cytokines in colonic tissue were observed within 24 hours.Results Compared with the normal group,the toxins in the model group were significantly increased at each time point(P<0.01),the Treg/Th17 cytokines were significantly increased(P<0.01),and the Treg/Th17 ratio was significantly lower(P<0.01).Compared with the model group,the expression of toxin and Treg/Th17-related cytokines were decreased in the clearing group,the warming group,the clearing and warming simultaneously group at 1 h,12 h,and 24 h(P<0.05).The decrease in the clearing and warming simultaneously group was the most significant(compared with the clearing group,the warming group,P<0.05).Compared with the model group,the ratio of Treg/Th17 was significantly increased in the clearing and warming simultaneously group than in the model group(P<0.05)Conclusion The mechanism of treating acute-on-chronic liver failure IETM in using clearing and warming simultaneously is related to the regulation of Treg/Th17 cytokine in colonic tissue and promoting the balance of Treg/Th17 cells.