湖南地区5种黄精属植物叶表皮的显微特征比较研究

Comparative Study on the Leaf Epidermis of Five Species of Polygonatum in Hunan Province

目的比较研究湖南地区黄精属5种植物的叶表面显微特征。方法采用剥撕法分别撕取叶上下表皮,用水合氯醛透化法制片,利用光学显微镜对植株成熟叶片中气孔的几项指标:气孔密度、气孔大小、气孔指数、气孔形态、气孔分布特点等进行观察;同时观察不同种植株的上下表皮细胞的异同。结果5种黄精属植物的叶表皮细胞存在一定的相似性,但在表皮细胞的大小、垂周壁的样式、气孔特征等方面存在着一定的差异。结论这5种植物的叶表面显微特征可作为湖南地区黄精属植物种间分类的参考依据。

Objective To comparatively study on leaf surface microscopic characteristics of 5 species of Polygonatum in Hunan province.Methods The upper and lower epidermis of leaves were separately torn by peeling method,making these tablets with Chloral Hydrate,and the stomatal indexes of mature leaves were observed by optical microscope,including stomatal density,stomatal size,stomatal index,stomatal morphology and stomatal distribution characteristics;the similarities and differences of the upper and lower epidermis cells of different plants were also observed at the same time.Results There were some similarities in leaf epidermal cells of 5 species of Polygonatum,but there were some differences in the size of epidermal cells,the pattern of vertical wall and stomatal characteristics.Conclusion The leaf surface microscopic characteristics of these 5 species can be used as a reference number for interspecific taxonomy of Polygonatum in Hunan province.