成纤维细胞生长因子23基因多态性与糖尿病肾脏疾病易感性的相关研究

Correlation of fibroblast growth factor 23 gene polymorphism with susceptibility of diabetic kidney disease

目的探讨陕西地区汉族人群中,成纤维细胞生长因子23(FGF-23)基因多态性与糖尿病肾脏疾病(DKD)的关系。方法选择2016年1月至2018年1月于榆林市中医医院住院治疗的214例病程超过5年的2型糖尿病(type 2 diabetes mellitus,T2DM)患者作为T2DM组,根据是否合并DKD将T2DM患者分为DKD组和non-DKD组两个亚组,另选择同期于我院行健康体检的80例健康志愿者作为对照组。采用ELISA法检测所有入组人员血清FGF-23水平;采用聚合酶链反应-直接测序法检测FGF-23基因rs7955866、rs13312756、rs3812822位点多态性。结果DKD组和non-DKD组血清FGF-23水平较对照组均显著增高,且DKD组显著高于non-DKD组(均P<0.01)。T2DM组与对照组比较,两组在rs7955866、rs13312756、rs3812822位点基因型和等位基因分布频率差异均无统计学意义(均P>0.05)。DKD组和non-DKD组比较,两组rs7955866位点基因型和等位基因频率分布频率差异均存在统计学意义(均P<0.01);两组rs13312756、rs3812822位点基因型和等位基因分布频率差异均无统计学意义(均P>0.05)。Logistics多因素回归分析结果显示携带rs7955866A等位基因是T2DM患者发生DKD的危险因素(OR=1.706,95%CI1.146~2.540,P=0.007)。结论陕西地区汉族人群中,FGF-23基因多态性可能与DKD临床易感性相关。

Objective To investigate the genetic association of fibroblast growth factor 23 (FGF-23) gene polymorphism with diabetic kidney disease (DKD) in the Han nationality population in Shanxi province.Methods A total of 214 type 2 diabetes mellitus (T2DM) patients with a disease course of over 5 years,hospitalized in YulinTraditional Chinese Medicine Hospital from January 2016 to January 2018,were recruited in this study as the T2DM group.According to whether complicated with DKD,they were divided into the DKD group and non-DKD group.The serum level of FGF-23 was measured with enzyme-linked immunosorbent assay (ELISA),and genotype was determined by polymerase chain reaction - directsequencing for the polymorphism of the FGF-23 gene loci rs7955866,rs13312756,rs3812822.Results The levels of serum FGF-23 of patients in the DKD group and non-DKD group were higher than that in the control group,and that in the DKD group was higher than that in the non-DKD group (allP<0.01).The genotype and allele frequencies at the loci rs7955866,rs13312756 and rs3812822 did not differ between the T2DM group and control group (allP>0.05).There were significantly difference of the genotype and allele frequencies of FGF-23 gene rs7955866 between the DKD group and non-DKD group (allP<0.01).However,the genotype and allele frequencies at the loci rs13312756 and rs3812822 did not differ between the DKD group and non-DKD group (allP>0.05).Multivariate Logistic regression analysis showed that carrying rs7955866 A allele was a risk factorfor DKD in T2DM patients ( OR =1.706,95% CI 1.146~2.540,P =0.007).Conclusions Polymorphism of FGF-23 gene may be correlated with DKD in the Han nationality population in Shanxi province.