软骨组织工程用羧甲基壳聚糖/氧化海藻酸钠复合水凝胶的制备及体外评估

Preparation and evaluation of carboxymethyl chitosan/sodium alginate hydrogel for cartilage tissue engineering

目的优化羧甲基壳聚糖/氧化海藻酸钠(CMCS/OSA)复合水凝胶制备工艺,并应用于软骨组织工程。方法制备氨基与醛基比例分别为2:1、1:1、1:2的CMCS/OSA复合水凝胶,并通过扫描电子显微镜观察、流变学检测、黏附拉力测试、溶胀率分析及细胞实验等方法评价复合水凝胶的微观形态、物理特性以及生物相容性,以制备满足软骨再生领域需求的水凝胶。结果氨基与醛基比为1:1时,制备出的水凝胶具有良好的孔隙率、适宜的成胶时间、较强的黏附力及稳定的溶胀率等性质,对细胞有良好的生物相容性。结论氨基与醛基比为1:1时制备出的CMCS/OSA复合水凝胶是软骨组织工程优异的支架材料。

Objective This study aimed to optimize the preparation of carboxymethyl chitosan/sodium alginate (CMCS/OSA) compound hydrogels. This study also aimed to investigate the applicability of the hydrogels in cartilage tissue engineering. Methods Three groups of CMCS/OSA composite hydrogels with amino-to-aldehyde ratios of 2:1, 1:1 and 1:2 were prepared. The microstructure, physical properties, and cell biocompatibility of the three groups of CMCS/OSA composite hydrogels were evaluated. Samples were subjected to scanning electron microscopy, rheological test, adhesion tension test, swelling rate test, and cell experiments to identify the CMCS/OSA composite hydrogel with the cross-linking degree that can meet the requirements for scaffolds in cartilage tissue engineering. Results The experimental results showed that the CMCS/OSA hydrogel with a amine-to-aldhyde ratio of 1:1 had good porosity, suitable gelling time, strong adhesive force, stable swelling rate, and good cellular biocompatibility. Conclusion The CMCS/OSA compound hydrogel prepared with a 1:1 ratio of amino and aldehyde groups has potential applications in cartilage tissue engineering.