槟榔碱及机械刺激构建大鼠口腔黏膜下纤维化模型

Rat model with oral submucous fibrosis induced by arecoline and mechanical stimulation

目的利用槟榔碱和机械刺激构建Sprague-Dawley(SD)大鼠口腔黏膜下纤维化(OSF)模型。方法采用两因素析因实验设计将48只大鼠分为8个组,每组6只。分别用不同浓度(0、0.5、2、8 mg·mL ^-1)槟榔碱涂擦及机械刺激(有或无毛刷涂擦)。处理16周后测量开口度;取局部颊黏膜行苏木精-伊红(HE)染色观察病理变化;并检测组织内Ⅲ型胶原、转化生长因子-β1(TGF-β1)和γ干扰素(IFN-γ)的表达情况。结果 2和8 mg·mL^-1(中、高)浓度槟榔碱处理16周,颊黏膜出现了典型的OSF病理特征;开口度显著减小并且Ⅲ型胶原、TGF-β1表达显著增加(P<0.05)。机械刺激虽可导致黏膜Ⅲ型胶原、TGF-β1和IFN-γ表达增高(P<0.05),但无病理学改变,开口度改变不显著。结论中、高浓度槟榔碱有致OSF作用;机械刺激无法导致大鼠OSF的发生。

Objective The aim of this study was to induce oral submucous fibrosis (OSF) in Sprague-Dawley(SD) rat models by arecoline and mechanical stimulation. Methods Two factors factorial design was used to divide 48 rats into 8 groups (n=6). Different concentrations of arecoline (0, 0.5, 2, and 8 mg·mL ^-1) and mechanical stimulation (with or without brush) were treated. After 16 weeks of treatment, the mouth opening was measured, the pathological changes of the buccal mucosa were observed, and the expressions of type Ⅲ collagen, transforming growth factor β1 (TGF-β1), and interferon-γ(IFN-γ) were detected. Results In rats with moderate and high concentrations of arecoline, typical OSF pathological features were observed in the buccal mucosa, the mouth openings were significantly reduced, and the expression levels of type Ⅲ collagen and TGF-β1 were significantly increased (P<0.05). Although mechanical stimulation can increase the three indexes of mucosa (P<0.05), no pathological change and difference in the mouth opening was observed (P>0.05). Conclusion Moderate and high concentrations of arecoline can induce OSF in SD rats, but mechanical stimulation cannot induce OSF.