血浆miRNA-320C和miRNA-6088对Ⅱ型糖尿病肾病的诊断价值

Diagnosis value of plasma miRNA-320C and miRNA-6088 in diagnosis of type Ⅱ diabetic nephropathy

目的研究miRNA-320C(miR-320C)和miRNA-6088(miR-6088)在Ⅱ型糖尿病肾病血浆中的表达,并探讨其对Ⅱ型糖尿病肾病的临床诊断价值。方法选取2015年6月至2016年12月在首都医科大学附属北京安贞医院就诊的56例Ⅱ型糖尿病(typeⅡdiabetic nephropathy,T2D)患者作为T2D组和105例Ⅱ型糖尿病肾病(diabetic nephropathy,DN)患者作为DN组,另选同时期体检的健康人52例作为正常对照组(normal control,NC)。实时荧光定量PCR检测miR-320C和miR-6088的表达。Pearson法分析miR-320C或miR-6088与其他临床指标之间的相关性,受试者工作特征(receiver operating characteristic,ROC)曲线分析miR-320C和miR-6088对Ⅱ型糖尿病肾病的诊断价值。结果miR-320C和miR-6088在DN组患者相对表达水平显著高于T2D组(P<0.05)和NC组(P<0.05)。Pearson相关分析显示,miR-C或miR-6088与尿蛋白排泄率(urinary albumin excretion rate,UAER)呈正相关,与肾小球滤过率(estimated glomerular filtration rate,eGFR)呈负相关,而与血浆糖化血红蛋白(human glycated hemoglobin A1C,HbA1C)、三酰甘油(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein-cholesterol,LDL-C)、糖尿病病程和体质量指数无相关性。ROC曲线分析miR-320C和miR-6088诊断Ⅱ型糖尿病肾病AUC分别为0.834和0.753。结论miR-320C和miR-6088在Ⅱ型糖尿病肾病患者外周血中高表达,是Ⅱ型糖尿病肾病患者诊断和预后的潜在标志物。

Objective To study the diagnosis value of plasma miRNA-320C(miR-320C)and miRNA-6088(miR-6088)in typeⅡdiabetic nephropathy(T2D).Methods Fifty-six T2D patients for T2D group,105 typeⅡdiabetic nephropathy(DN)for DN group and 52 healthy people for normal control(NC)group were recruited from Jun.2015 to Dec.2016 in Beijing Anzhen Hospital,Capital Medicical University.The expression of circulating miR-320C andmiR-6088 were detected by RT-qPCR.Pearson analysis was used to analyze the correlation between miR-320C or miR-6088 and clinical data.And receiver operating characteristic(ROC)curve was drawn to test the diagnostic value of miR-320C and miR-6088.Results The expression of circulating miR-320C and miR-6088 in DN group was higher than NC group(P<0.05)and T2D group(P<0.05).Pearson correlation analysis showed that miR-320C or miR-6088 was positively correlated with urinary albumin excretion rate(UAER),negatively correlated with estimated glomerular filtration rate(eGFR),while had no correlation with the plasma levels of human glycated hemoglobin A1C(HbA1C),triglycerides,low density lipoprotein-cholesterol(LDL-C)and diabetes duration,body mass index.ROC curves of miR-320C and miR-6088 presented an area under curve of 0.834 and 0.753,respectively.After treatment,both miR-320C and miR-6088 expression were significantly decreased(P<0.05).Conclusion Circulating miR-320C and miR-6088 were highly expressed in typeⅡdiabetic nephropathy,which may serve as useful diagnostic and prognostic biomarkers with typeⅡdiabetic nephropathy.