人参二醇组皂苷对四氯化碳所致大鼠急性肝损伤的保护作用及其机制

Protective effect of panaxdiol on acute liver damage of rats induced by carbon tetrachloride and its mechanism

目的:探讨人参二醇组皂苷(PDS)对四氯化碳(CCl4)所致急性肝损伤(ALD)大鼠的保护作用,并阐明其作用机制。方法:50只大鼠随机分为对照组(给予生理盐水),模型组(制备ALD模型,给予生理盐水),低、中和高剂量PDS组(制备ALD模型,给予10、20和40mg·kg^-1PND),每组10只。观察各组大鼠一般状态;检测各组大鼠谷氨酸氨基转氨酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBIL)和总蛋白(TP)水平;ELISA法测定各组大鼠肝组织匀浆中白细胞介素12 (IL^-12)、白细胞介素6 (IL-6)和肿瘤坏死因子α(TNF-α)水平;Western blotting法检测各组大鼠肝组织中蛋白激酶B (又称AKT)和磷酸化蛋白激酶B (p-AKT)蛋白表达水平。结果:与对照组比较,模型组大鼠一般状态差,ALT、AST和TBIL水平明显升高(P<0.01),TP水平降低(P<0.01),IL^-12、IL-6和TNF-α水平均明显升高(P<0.01)。与模型组比较,各剂量PDS组大鼠肝功能明显改善,ALT、AST和TBIL水平降低(P<0.01),TP水平升高(P<0.01),IL^-12、IL-6和TNF-α水平降低(P<0.01),肝组织中AKT蛋白表达水平降低(P<0.01),p-AKT蛋白表达水平升高(P<0.01)。结论:PDS对ALD具有保护作用,其机制可能与降低炎症因子的表达、影响p-AKTAKT信号通路有关。

Objective:To investigate the protective effect of panaxdiol saponins(PDS)on the acute liver damage(ALD)of the rats induced by carbon tetrachloride(CCl4),and to clarify its mechanism.Methods:Fifty rats were randomly divided into control group(the rats were given normal saline),model group(the ALD models were established and given normal saline)and low,middle,and high doses(10,20,and 40 mg·kg^-1)of PDS groups,and there were 10 rats in each group.The general state of the rats in various groups was observed.The levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),and total protein(TP)of the rats in various groups were detected;ELISA method was used to detecet the levels of interleukin-6(IL-6),interleukin-12(IL^-12)and tumor necrosis factor-α(TNF-α)in liver tissue homogenate of the rats in various groups;Western blotting method was used to detecet the expression levels of protein kinase B(AKT)and p-protein kinase B(p-AKT)in liver tissue of the rats in various groups.Results:Compared with control group,the levels of ALT,AST and TBIL of the rats in model group were increased(P<0.01),and the level of TP was decreased(P<0.01);the levels of IL^-12,IL-6 and TNF-α were increased(P<0.01).Compared with model group,the hepatic function of the rats in different doses of PDS groups were obviously improved,the levels of ALT,AST and TBIL in liver tissue of the rats in different doses of PDS groups were decreased(P<0.01),the levels of TP were increased(P<0.01);the levels of IL^-12,IL-6 and TNF-αin liver tissue of the rats were decreased(P<0.01);the expression levels of AKT protein in liver tissue of the rats were decreased(P<0.01)and the expression level of p-AKT was increased(P<0.01).Conclusion:PDS has the protective effect on ALD,and its mechanism may be related to reducing the expressions of inflammatory factors and affecting the p-AKT-AKT signal pathway.