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UPLC/MS/MS法定性定量测定中药材中3类27种合成色素 被引量:7

Qualitative and Quantitative Determination of 27 Synthetic Dyes of 3 Species in Herbs by UPLC/MS/MS
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摘要 目的:采用液相-固相萃取-超高效液相色谱-电喷雾串联质谱联用技术对27种色素建立了准确、灵敏的分析方法。方法:针对脂溶性、酸性和碱性3类色素分别采用不同的制备方法、色谱条件和电喷雾极性,采用4种不同药用部位的代表性药材进行了方法学考察。结果:表明方法线性关系良好(r≥0. 997);脂溶性和碱性色素的检测限为0. 03~3. 87 ng/g,酸性色素为0. 003~1. 94μg/g;准确度和精密度分别为70%~105%和0. 9%~6. 8%;应用于25个品种80批样品的筛查。结论:所建方法可靠、高效、准确,适用于中药材复杂基质中不同种类色素的测定。 Objective: To develop a sensitive and accurate method combining liquid and solid phase extraction with ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry to analyze 27 dyes. Methods: Different preparations,chromatography conditions and polarity of ESI were applied for 3 categories of lipophilic,acidic and basic dyes respectively.Four species of herbs representing different parts of plant were selected for methodology observation. Results: The linear correlation coefficient was good(r ≥ 0.997).The LODs of oil soluble and basic dyes were 0.03~3.87 ng/g and acid dyes were 0.003~ 1.94 μg/g respectively.The accuracies and precisions were 70%~105% and 0.9%~6.8% respectively. Conclusion: After detecting 80 samples of 25 species herbs,this method proved to be reliable,efficient and accurate in the detection of different kinds of dyes in the complex matrix of herbs.
作者 张甦 胡青 孙健 冯睿 于泓 季申 Zhang Su;Hu Qing;Sun Jian;Feng Rui;Yu Hong;Ji Shen(Shanghai Institute for Food and Drug Control,Shanghai 201203,China)
出处 《世界中医药》 CAS 2019年第4期822-827,832,共7页 World Chinese Medicine
基金 中国药典2015年版标准提高方法研究项目(中药中常用色素检测方法的研究) 上海市科委技术服务平台(18DZ2292200)
关键词 27种 合成色素 脂溶性 酸性 碱性 定性定量 中药材 UPLC/MS/MS 27 kinds Artificial dyes Lipophilic,Acidity Basicity Qualitative and quantitative determination Herbs UPLC/MS/MS
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