摘要
目的:研究扶正解毒化瘀方对多重耐药铜绿假单胞菌MexAB-OprM外排泵表达的影响。方法:针对筛选出的MexAB-OprM阳性的铜绿假单胞菌菌株,通过微量稀释法,测定哌拉西林-他唑巴坦的最小抑菌浓度和扶正解毒化瘀方水提物的最小杀菌浓度,从而确定荧光定量PCR细菌样本时培养剂中哌拉西林-他唑巴坦和扶正解毒化瘀方水提物的浓度。绘制细菌生长曲线,确定细菌样本的培养时间。利用实时荧光定量PCR技术检测药物干预前后mex B、oprM、mexR的表达量。结果:扶正解毒化瘀方水提物能降低MexAB-OprM外排泵阳性MDRPA菌株的mex B、oprM表达量(P <0. 05),同时能增加mexR的表达量(P <0. 05)。结论:扶正解毒化瘀方对MDRPA所致肺炎的作用机制之一可能是通过抑制MexAB-OprM外排泵的表达实现的。
Objective: To study the effects of Fuzheng Jiedu Huayu Decoction on the expression of MexAB-OprM efflux pumps in multidrug-resistant Pseudomonas aeruginosa. Methods: The minimum inhibitory concentration of piperacillin-tazobactam and the minimum bactericidal concentration of the water extract of Fuzheng Jiedu Huayu Decoction were determined via microdilution method for selected MexAB-OprM positive Pseudomonas aeruginosa strains so as to determine the concentration of piperacillin-tazobactam and the water extract of Fuzheng Jiedu Huayu Decoction in the culture medium during the fluorescence quantitative PCR of bacterial samples.The bacterial growth curve was drawn and the culture time of the bacterial samples was determined.The expression levels of mexB,oprM,and mexR before and after drug intervention were detected by real-time fluorescence quantitative PCR technology. Results: The expression levels of mexB and oprM in MexB-OprM efflux pump positive strains were decreased after the intervention of Fuzheng Jiedu Huayu Decoction water extract( P <0.05),and that of mexR was increased than before( P <0.05). Conclusion: The therapeutic effect of Fuzheng Jiedu Huayu Decoction on pneumonia caused by MDRPA may be attributed to the inhibition of the expression of MexAB-OprM efflux pump.
作者
王玉婷
徐红日
赵世同
程淼
姚兴伟
常桂娇
陈师林
王成祥
Wang Yuting;Xu Hongri;Zhao Shitong;Cheng Miao;Yao Xingwei;Chang Guijiao;Chen Shilin;Wang Chengxiang(Respiratory Department,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Emergency Department,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China;Clinical Laboratory Department,Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;Respiratory Department,Beijing University of Chinese Medicine Third Affiliated Hospital,Beijing 100029,China)
出处
《世界中医药》
CAS
2019年第4期859-863,共5页
World Chinese Medicine
基金
国家自然科学基金面上项目(81573924)
