PCR检验法与细菌培养法用于阴道细菌检验的效果对比

Comparison of the effect of PCR test and bacterial culture test on vaginal bacteria test

目的探究PCR检验法(聚合酶链反应检测)与细菌培养法用于阴道细菌检验的效果对比。方法选取2017年9月至2018年1月本院收治的240例细菌性阴道炎患者,符合临床诊断标准,分别采用PCR检验法与细菌培养法检测其阳性率和致病菌种类。结果PCR检验法检出阳性率为40.4%,细菌培养法检出阳性率为30.8%,两组患者比较差异有统计学意义(P<0.05)。PCR检验法检出97例阳性患者中,有25例棒状杆菌患者、35例肠球菌患者、37例加特纳菌患者。细菌培养法检出74例阳性患者中,有22例棒状杆菌患者、21例肠球菌患者、31例加特纳菌患者。结论PCR检验法较之细菌培养法在阴道细菌检验中,阳性检测率较高,可有效检查致病菌,值得临床阴道细菌检测使用。

Objective To explore the effect of PCR test (polymerase chain reaction test) and bacterial culture on vaginal bacteria test. Methods 240 cases of bacterial vaginitis admitted to our hospital from September 2017 to January 2018 were selected, which met the clinical diagnostic criteria. The positive rate and pathogenic bacteria species were detected by PCR test and bacterial culture respectively. Results The positive rate was 40.4% by PCR and 30.8% by bacterial culture, and the difference between the two groups was statistically significant (P<0.05). Among the 97 positive cases detected by PCR, there were 25 cases of corynebacterium, 35 cases of enterococcus and 37 cases of gastrium. Among the 74 positive cases detected by the bacterial culture method, there were 22 cases of corynebacterium, 21 cases of enterococcus and 31 cases of gastrium. Conclusion Compared with the bacterial culture method, the PCR method has a higher positive detection rate, which can effectively detect pathogenic bacteria and is worthy of clinical vaginal bacteria detection.