内镜超声引导下钬激光对裸鼠胰腺原位种植瘤消融效果的实验研究

Therapeutic effects of endoscopic ultrasound-guided holmium laser ablation for pancreatic primary implantation carcinoma in nude mice

目的探索内镜超声引导下钬激光对裸鼠胰腺原位种植瘤消融治疗的有效性,为内镜超声引导下钬激光对肿瘤的消融治疗应用于临床提供实验基础。方法选取4~6周龄的雄性balb/c裸鼠20只,将胰腺癌细胞株SW1990接种至裸鼠胰腺,构建裸鼠原位种植瘤模型,随机分配裸鼠成2组,每组10只,实验组给予内镜超声引导下钬激光的消融治疗,对照组不给予任何治疗。于治疗后第7天,第14天和第28天予内镜超声下测量实验组及对照组裸鼠肿瘤的大小,观察裸鼠活动、食欲等变化。解剖裸鼠,取出肿瘤,HE染色显微镜下观察胰腺肿瘤组织的病理变化。结果裸鼠均可耐受内镜超声引导下钬激光的消融治疗,实验组裸鼠术后无明显不适反应,精神状态、活动量及食欲较好,对照组裸鼠精神状态逐渐恶化,体形消瘦。消融后28 d实验组裸鼠肿瘤体积缩小,对照组裸鼠肿瘤增大,两组肿瘤体积的变化差异有统计学意义。钬激光治疗疗效大约持续28 d,HE染色可见消融部位呈凝固性坏死。结论内镜超声引导下钬激光对裸鼠胰腺原位种植瘤的消融治疗有效,疗效大约持续28 d,主要引起肿瘤组织的凝固性坏死。

Objective To explore the effectiveness of endoscopic ultrasound(EUS)-guided holmium laser ablation for primary pancreatic implantation tumor in nude mice. Methods Pancreatic cancer cell line SW1990 were implanted into 20 4-6-week-old male balb/c nude mice to establish primary pancreatic implantation tumor in situ models. Then the nude mice were randomly divided into two groups, the treatment group(n=10) and the control group(n=10). The treatment group underwent EUS-guided holmium laser ablation in the pancreatic tumor. And no interventions were given to the control group. The volume of tumors of the two groups were measured under EUS at time points of 7 d, 14 d and 28 d after ablation. The activities, appetites and psychosis of all nude mice were evaluated in the meantime. At 28 d after ablation, lesions of pancreas were dissected and sliced for H&E staining. Results There were no complications in the treatment group, and all nude mice could tolerate the procedure. The mental state, activities and appetites of nude mice in the experimental group were better than those in the control group. Tumors of the control group enlarged. There was significant difference in the tumor size between the two groups at 28 d after ablation. HE staining showed coagulation necrosis in the ablation area. Conclusion EUS-guided holmium laser, producing coagulative necrosis in the ablation area, is effective for primary pancreatic implantation carcinoma in nude mice for about 28 days.