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以壳聚糖金纳米粒子为载体构建Ch- GNPs/β6复合物的相关研究 被引量：2

A study on the construction of Ch-GNPs/β6 complex with chitosan gold nanoparticles as carrier
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摘要 通过凝聚过程形成Ch-GNPs,以其为载体,搭载整合素β6质粒,形成Ch-GNPs/β6复合物。利用紫外可见分光光度计,透射电子显微镜(TEM)检测复合物的性状。进一步通过电泳、WB和X-gal实验检测复合物的结合力及对MC3T3-E1细胞的转染能力。结果表明Ch-GNPs/β6复合物性状稳定并能有效在MC3T3-E1细胞中进行转染。 The Ch-GNPs were conjugated with the pcDNA-β6 through a coacervation process. As the carrier, with integrin αvβ 6 plasmid, Ch-GNPs/β 6 compound was formed. The size and surface characterization of Ch-GNPs were examined using UV-vis spectroscopy and TEM. The DNA conjugation and transfection capacity of Ch-GNPs were studied by agarose gel electrophoresis, β-galactosidase staining and immunoblotting.The study suggests that the chitosan and the acid chloride alloy through the simple condensation process can form Ch-GNPs compounds.The Ch-GNPs with the right granularity can be transfected into MC3 T3-E1 cells.
作者 潘夏薇 赖颖真 许铭炎 PAN Xiawei;LAI Yingzhen;XU Mingyan(Xiamen Medical College,361000 China;Xiamen Stomatological Hospital)
机构地区 厦门医学院口腔医学系 厦门医学院附属口腔医院
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2019年第3期461-463,共3页 Journal of Practical Stomatology
基金 2015年福建省卫生系统中青年骨干人才培养项目(编号:2015-ZQN-ZD-35) 厦门医学高等专科学校自然科学基金(编号:K2015-06)
关键词 壳聚糖 金纳米粒子 整合素ΑVΒ6 Chitosan Gold nanoparticles Integrin αvβ6
分类号 R783.1 [医药卫生—口腔医学]
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实用口腔医学杂志
2019年 第3期

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