温针灸对急性痛风性关节炎家兔血清中TNF-α和IL-1、IL-8表达的影响

The Effect of Needle Warming Therapy on TNF-α、IL-1 and IL-8 of Serum of Gouty Srthritis Rabbit Model

目的:观察温针灸对急性痛风性关节炎家兔血清TNF-α、IL-1、IL-8含量的影响。方法:将40只wist家兔随机分为空白组、模型组、西药组和温针灸组,每组10只。除空白组常规喂养外,其余各组将尿酸钠溶液通过髌上韧带注入家兔膝关节,造成急性痛风性关节炎变病模型。造模结束后,空白组和模型组不作任何处理,西药组造模后予以秋水仙碱悬浮液灌胃治疗7天,温针灸组取家兔右侧"太冲""三阴交""合谷"治疗7天。观察家兔造模前后的皮色、皮温、精神状态、步态、饮食情况,采用ELISA法检测各组家兔血清中的TNF-α、IL-1、IL-8含量。结果:与正常组相比,模型组家兔关节严重跛行,血清中IL-1、IL-8和TNF-α含量明显高于空白组(P<0.05);与模型组相比,西药组和温针灸组家兔IL-1、IL-8和TNF-α的含量明显下降(P<0.05)。结论:温针灸对急性痛风性关节炎具有一定的治疗作用,其发挥抗炎作用机制可能与其抑制血清中IL-1、IL-8和TNF-α的表达有关。

Objective: To observe the levels of tumor necrosis factor(TNF-α), interleukin(IL)-1 and IL-8 in serum of rabbits with acute gouty arthritisthe after treatment of warm acupuncture.Methods: Forty rabbits were randomly divided into four groups,control group, model group, medication group and warm acupuncture group,with 10 rabbits in each group.Regular feeding was used in the blank group, the other groups were injected with sodium urate solution through the patellar ligament into the knee joint of rabbits to form model group. The rabbits were treated with physiological saline and sodium urate solution for control group and model group,respectively. At same time no warm acupuncture were given.The model of acute gouty arthritis was established in the model group, medication group and warm acupuncture group. The rabbits in the medication group were given colchicine gavage administration for 7 days.Warm acupuncture was applied to "Taichong""San yin jiao" and "Hegu" for 7 days.The degree of foot swelling was measured,and the color,and temperature of skin,mental state,gait and diet in each group were observed. The levels of tumor necrosis factor(TNF-α), interleukin(IL)-1 and IL-8 in serumwere detected by Elisa assay,respectively. Results: Compared with control group,the state of the swelling was worse in the model group,while the state of the swelling was better in both medication and warm acupuncture groups.The concentrations of serum IL-1, IL-8 and TNF-α were all significantly higher in the model group than in the control group(P< 0.05),but those of serum IL-1, IL-8 and TNF-α in both medication group and warm acupuncture group were significantly down-regulated following the intervention(P<0.05). Conclusion: Warm acupuncture attenuated inflammatory responses through reduction levels of IL-1, IL-8 and TNF-α in serum of rabbits with acute gouty arthritis.