HPLC法测定肉苁蓉水提液中松果菊苷的含量

Determination of Echinacoside in Water Extract of Cistanche Herba by HPLC

目的建立HPLC法测定肉苁蓉水提液中松果菊苷的含量。方法采用HPLC法测定松果菊苷的含量,色谱柱为Inertsil ODS-SPC18柱;流动相为乙腈(A)-0.4%磷酸溶液(B),梯度洗脱;检测波长为333nm;柱温为30℃:;流速为1.0mL·min^-1;进样量为10μL。结果松果菊苷在44.9397~539.2764μg·ml^-1的浓度范围内线性关系良好(r=0.9998);加样回收率为94.59%~105.31%(RSD=2.93%,n=9);3批肉苁蓉水提液中松果菊苷的平均含量分别为15.527、7.347、12.267mg·g^-1。结论所建立的HPLC法准确、重复性好、简单易行,适用于肉苁蓉水提液中松果菊苷的含量测定。

Objective An HPLC method was established for determination of echinacoside in water extract of Cistanche herba.Methods Echinacoside was determined by HPLC.The separation was performed on Inertsil ODS-SP C18 column with mobile phase consisted of acetonitrile (A) and 0.4% phosphoric acid solution (B),by gradient elution.The detection wavelength was set at 333 nm,and the column temperature was 30 ℃.The flow rate was 1.0 mL·min^-1 and the injection volume was 10 μL.Results The linear relation of echinacoside within the concentration range of 44.939 7-539.276 4 μg·mL^-1 was good (r = 0.999 8).The recoveries were 94.59%-105.31%(RSD=2.93%,n = 9).The content of echinacoside in water extracts of Cistanche herba was 15.527,7.347,12.267 mg·g^-1.Conclusion The established HPLC method with good repeatability is accurate,simple and feasible.It is suitable for the determination of echinacoside in water extract of Cistanche herba.