何首乌有效成分对大鼠骨癌痛的镇痛效果

Analgesic effect of active ingredients of polygonum multiflorum on bone cancer pain in rats

目的探讨何首乌有效成分对大鼠骨癌痛的镇痛作用。方法将36只SPF级雌性SD大鼠随机分成3组:A组为正常空白对照(n=6),B组大鼠注射无菌生理盐水(n=6),C组大鼠左后腿胫骨注射乳腺癌细胞walker256建立骨癌痛模型(n=24)。建立骨癌痛模型的C组SD大鼠按灌胃药物随机分成C1、C2、C3、C4 4组,每组6只。建模成功后14 d、15 d、16 d连续对C1~C4组大鼠分别灌胃生理盐水(2 mL)、二苯乙烯苷(60 mg/mL,2 mL)、大黄素(60 mg/mL,2 mL)和儿茶素(60 mg/mL,2 mL)。每次灌胃前、灌胃后30 min、灌胃后1.0 h测量大鼠左脚掌的机械痛阈值。结果癌痛模型喂养第5天起,与A、B两组比较,C组大鼠机械痛阈值降低(P<0.05),骨质明显破坏;A、B两组大鼠机械痛阈值无明显差异,骨质结构正常。C2组大鼠各时点的机械性痛阈值均较C1组、C3组、C4组明显增加(P<0.05)。与灌胃前比较,C2组大鼠模型灌胃30 min和灌胃后1 h后机械性痛阈值明显升高(P<0.05),但灌胃30 min和灌胃后1 h后的机械性痛阈值无统计学意义(P>0.05)。结论何首乌可有效降低大鼠的骨癌痛,其有效单体成分是二苯乙烯苷。

Objective To explore the analgesic effect of active ingredients of polygonum multiflorum on bone cancer pain in rats. Methods Specific pathogen-free female Sprague-Dawley rats were randomly divided into 3 groups: blank control group(A group,n=6),modeled as sterile saline group(B group,n=6),and bone cancer pain group(C group,n=24). Rats in B group were given sterile saline during establishment of model while those in C group were injected with breast cancer cells (walker256) at the upper part of the left hind leg to establish a model with bon cancer pain. Those rats in C group were randomly divided into C1,C2,C3 and C4 groups according to the species of drugs administered intragastrically(n=6). After successful modeling 14 d,15 d and 16 d,rats in C1~C4 group were intragastrically administered with normal saline(2 mL),tetrahydroxystilbene glucoside(60 mg/mL,2 mL),emodin(60 mg/mL,2 mL) and catechin(60 mg/mL,2 mL) respectively. The pain threshold of left foot in each group was measured before each gavage,30 min after gavage,1h after gavage. Results From the 5th day of feeding in rats with cancer pain model,the mechanical pain threshold in C group was significantly decreased and sclerotin structure were obviously destructed compared with A and B groups(P<0.05),but there were no statistical difference observed on the pain threshold and sclerotin structure between A and B groups. Compared with C1,C3 and C4 group,the mechanical pain threshold at each time point in C2 group was significantly increased(P<0.05). In the C2 group,pain threshold after 30 min and 1 h of gavage were both signifi-cantly increased than that before gavage(P<0.05);but the pain threshold after 30 min and 1 h of gavage were similar(P>0.05). Conclusion Polygonum multiflorum can effectively attenuate bone cancer pain in rats and its active ingredients was tetrahydrox-ystilbene glucoside.