重症急性胰腺炎大鼠伴发肠黏膜屏障损伤和Occludin蛋白表达变化

Intestinal mucosal barrier injury and Occludin protein expression in rats with severe acute pancreatitis

目的探讨重症急性胰腺炎(SAP)伴发肠黏膜屏障的损伤及Occludin蛋白表达变化。方法将16只SD大鼠随机分为对照组(假手术)、模型组(建模手术),每组8只。SAP大鼠建模参照Schmidt方法。手术后24h处死大鼠,尾静脉采血,ELISA法检测血清内毒素;取肠组织进行形态学检查,并进行结肠损伤评分。采用免疫荧光定位Occludin蛋白在肠上皮的表达。结果模型组内毒素水平[(0.280±0.002)Eu/L]较对照组[(0.082±0.004)Eu/L]明显升高,两组比较有统计学差异(t=125.23,P<0.01)。模型组结肠组织损伤病理学评分较对照组明显升高[(6.3±1.0)分vs(2.9±0.8)分,t=7.509,P<0.01]。对照组结肠黏膜细胞浆Occludin表达灰度值正常(30.1±2.7),而模型组Occludin表达灰度值明显减少(19.0±0.6),两组比较差异有统计学意义(t=11.351,P<0.01)。结论SAP多伴有肠黏膜损伤,同时伴有肠上皮Occludin蛋白表达减少。

Objective To investigate the intestinal mucosal barrier injury and the expression of Occludin protein in rats with severe acute pancreatitis ( SAP ).Methods Sixteen SD rats were randomly divided into control group ( sham operation) and model group( modeling operation),with 8 rats in each group.The model of SAP was established by Schmidt method.Rats were executed 24 hours after operation, blood was collected from caudul vein, and serum endotoxin was detected by ELISA.The intestinal tissues were taken for morphological examination and colonic injury score was performed.Immunofluorescence was used to localize Occludin protein expression in the intestinal epithelium.Results The endotoxin level in the model group [( 0.280 ± 0.002) Eu /L] was significantly higher than that in the control group [( 0.082 ± 0.004) Eu /L].There was significant difference between the two groups ( t = 125.23, P < 0.01).The injury pathological score of colon tissue in the model group was significantly higher than that in the control group [( 6.3 ± 1.0) vs ( 2.9 ± 0.8),t = 7.509,P < 0.01].The gray value of Occludin expression in the colonic mucosa of the control group was normal ( 30.1 ± 2.7),while that in the model group was significantly decreased ( 19.0 ± 0.6).The difference between the two groups was statistically significant ( t = 11.351, P < 0.01).Conclusion SAP is often associated with intestinal mucosal injury,and accompanied by decreased expression of Occludin protein in the intestinal epithelium.