慢性阻塞性肺疾病患者血清IL-33、IFN-γ表达与Th1/Th2平衡失调

Expressions of IL-33 and IFN-γ in serum and imbalance of Th1/Th2 in patients with chronic obstructive pulmonary disease

目的探讨白介素-33(IL-33)、干扰素-γ(IFN-γ)在慢性阻塞性肺疾病(COPD)患者血清中的表达水平及其临床意义,观察COPD患者辅助T细胞(Th)1/Th2平衡失调特点。方法选择2017年2月至2018年4月在昆明医科大学第一附属医院就诊的110例COPD患者作为研究对象,其中急性加重期55例(急性加重组),稳定期55例(稳定组),另外选择30例健康体检者作为对照组。采用酶联免疫法分别检测血清中IL-33、IFN-γ的水平,并分析急性加重期和稳定期COPD患者的Th1/Th2状态。结果COPD急性加重组和稳定组患者血清中IL-33、IFN-γ水平均高于对照组(P<0.05),急性加重组患者血清中IL-33、IFN-γ水平均高于稳定组(P<0.05)。COPD急性加重组IFN-γ/IL-33比值低于对照组和稳定组(P<0.05),而稳定组IFN-γ/IL-33比值高于对照组(P<0.05)。结论IL-33与IFN-γ共同参与了COPD气道炎症发生及发展过程。COPD患者存在Th1/Th2免疫平衡失调。

Objective To investigate the serum expressions and clinical significance of interleukin-33 ( IL-33 ) and interferon gamma ( IFN-γ) in patients with chronic obstructive pulmonary disease ( COPD) and the characteristics of T helper cell ( Th) 1 /Th2 imbalance in COPD patients.Methods A total of 110 patients with COPD treated in the First Affiliated Hospital of Kunming Medical University from February 2017 to April 2018 were selected as the study subjects and divided into acute exacerbation group and stable stage group( n = 55, each).Another 30 heathy people were enrolled as control group.The serum levels of IL-33 and IFN-γ were detected by enzyme linked immunosorbent assay ( ELISA), and Thl /Th2 status of different COPD stages were analyzed.Results The serum levels of IL-33 and IFN-gamma in acute exacerbation group and stable group were significantly higher than those in control group ( all P < 0.05 ) and were significantly higher in acute exacerbation group than those in stable group ( P < 0.05).The ratio of IFN-gamma /IL-33 in acute exacerbation group was significantly lower than that in control group ( P < 0.05),while it was significantly higher in stable group than that in control group ( P < 0.05).Conclusions IL-33 and IFN-gamma participate in the occurrence and development of airway inflammation in COPD.Th1 /Th2 cytokine imbalance exists in COPD patients.