在线固相萃取-高效液相色谱法同时测定眩晕停胶囊中的葛根素和芍药苷

Simultaneous determination of puerarin and paeoniflorin in Xuanyunting Capsules by online solidphase extraction coupled with HPLC

采用固相萃取-高效液相色谱法建立在线同时测定眩晕停胶囊中葛根素和芍药苷含量的方法.通过Online SPE方法可以有效去除样品中其他成分的干扰,葛根素的质量浓度在44.00-132.00μg·mL-1(r=0.9997)范围内同相应峰面积呈良好的线性关系,加标回收率为97.07%,相对标准偏差RSD为0.98%.芍药苷的质量浓度在284.00-852.00μg·mL^-1(r=0.9991)范围内同相应峰面积呈良好的线性关系,加标回收率为103.64%,RSD为0.51%.

A method for Simultaneous determination of puerarin and paeoniflorin in XuanyuntingCapsules by online solidphase extraction coupled with HPLC. The calibration curve of puerarin wasliner within the range of 44.00-132.00 μg·mL-1(r=0.9997).The average recovery was 97.07%(n=6) and RSD was 1.24%.The calibration curve of paeoniflorin was liner within the range of 284.00-852.00 μg·mL^-1(r=0.9991). The average recovery was 103.64%(n=6) and RSD was 0.501%.