管氏肿腿蜂寄生对松墨天牛幼虫体质量和营养物质含量的影响

Effects of parasitization by Sclerodermus guani (Hymenoptera: Bethylidae) on body weight and nutrient content of Monochamus alternatus (Coleoptera: Cerambycidae) larvae

管氏肿腿蜂是多种林木钻蛀性害虫的抑性寄生蜂,雌蜂通过蜇刺抑制寄主氧化反应。为明确肿腿蜂寄生后寄主体内营养物质含量的变化,运用考马斯亮蓝法、蒽酮比色法和残余法,对蜇刺后第0日(初始组)、第2日(寄主初麻痹时)、第14日(子代幼蜂初孵时)处理组和对照组的松墨天牛幼虫体质量、蛋白质、总糖和脂质含量进行测定。结果表明:管氏肿腿蜂寄生后,寄主体质量持续下降;体内蛋白质含量在寄主麻痹初期极显著升高,而在幼蜂初孵时则极显著下降;脂质含量在寄主初麻痹时未见显著变化,而幼蜂孵化前升高显著;但寄主体内的总糖含量变化不显著。研究结果说明,管氏肿腿蜂雌蜂寄生寄主,对寄主体内蛋白质、糖类、脂质代谢产生了影响。

Sclerodermus guani is idiobiont parasitoid of many wood-boring insects.The females inject venom and ovarian proteins by sting to inhibit oxidation of host insect.To investigate effects of parasitization by S.guani on nutrient content of Monochamus alternatus host larvae,the total protein,lipid and total carbohydrate contents of the healthy and parasitized host insects on day 0,2 and 14 were examined using Bradford s method,Anthrone colorimetry and the residue weight method,respectively.The results were as follows:After parasitization,the weight of M.alternatus larvae decreased consistently;Protein contents of M.alternatus increased significantly on day 2,but significantly reduced on day 14;No significant change in lipid contents of M.alternatus occured on day 2,but increased significantly on day 14;Total carbohydrate contents had no difference between healthy and parasitized host larvae consistently.The results suggested that S.guani could regulate the metabolism of protein,lipid and carbohydrate of host larvae after parasitization,which would provide a basis for further studies on the mechanisms of nutritional metabolism of the host regulated by S.guani.