楸叶泡桐杂种无性系9503的组织培养技术研究

Research of tissue culture in hybrid clone 9503 of Paulownia catalpifolia

以楸叶泡桐和白花泡桐杂交无性系的枝条为外植体,开展了离体芽增殖技术,以及愈伤组织诱导、芽分化和不定芽生根等组织培养技术的研究。结果表明,增殖培养基采用MS+8mg/L6-BA+0.3mg/LNAA,不定芽的增殖倍数可达到9;利用其叶片进行愈伤组织诱导的最佳培养基为MS+12mg/L6-BA+0.4mg/LNAA,诱导率高达95%;愈伤组织分化的最佳培养基为MS+10mg/L6-BA+0.7mg/LNAA,不定芽生根的最佳培养基为1/2MS+0.5mg/LIBA+0.3mg/LNAA,生根率100%。

By using excellent stem segments of the hybrid Clone 9503 of Paulownia fortunei and P.catalpifolia as explants,we studied the tissue culture technology,including callus induction,bud differentiation and bud proliferation.The results showed that the multiplication medium was MS+8 mg/L 6-BA+0.3 mg/L NAA,with 9 of multiplication time.The suitable medium for leaf callus induction was MS+12 mg/L 6-BA+0.4 mg/L NAA,with the induction rate of 95%.The suitable callus differentiation medium was MS+10 mg/L 6-BA+0.7 mg/L NAA.The suitable rooting culture medium was 1/2MS+0.5 mg/L IBA+0.3 mg/L NAA,with the rooting rate of 100%.