不同来源酒曲酿制米酒中乳酸菌的分离与鉴定

Isolation and identification of lactic acid bacteria from rice wine fermented by Jiuqu from different sources

为了研究湖北孝感和四川成都地区米酒曲中乳酸菌的多样性,该研究利用孝感凤窝米酒曲和成都农家自制米酒曲制作米酒,并采用改良MRS固体培养基和分子生物学技术对米酒中的乳酸菌进行分离、鉴定。结果表明,从孝感米酒中共分离出42株乳酸菌,隶属于7个种,分别为戊糖片球菌(Pediococcus pentosaceus)、融合魏斯氏乳酸菌(Weissella confusa)、植物乳杆菌(Lactobacillus plantarum)、发酵乳杆菌(Lactobacillus fermentum)、能动乳杆菌(Lactobacillus agilis)、屎肠球菌(Enterococcus faecium)和乳酸乳球菌(Lactococcus lactis);从成都米酒中共分离出35株乳酸菌,隶属于7个种,分别为P. pentosaceus、W. confusa、L. plantarum、E. faecium、食窦魏斯氏乳酸菌(Weissella cibaria)、约汉逊氏乳杆菌(Lactobacillus johnsonii)和短乳杆菌(Lactobacillus brevis)。两种米酒中分离出的相同菌种为P. pentosaceus、W. confusa、L. plantarum和E. faecium。其中,P. pentosaceus为两种米酒的优势菌株。

In order to research the diversity of lactic acid bacteria in rice wine Jiuqu from Xiaogan, Hubei and Chengdu, Sichuan, the rice wine was fermented by Xiaogan Fengwo rice wine Jiuqu and Chengdu farmer homemade rice wine Jiuqu. The lactic acid bacteria in rice wine were isolated and identified by improved MRS solid medium and molecular biology technology. The results showed that 42 strains of lactic acid bacteria were isolated from Xiaogan rice wine, which belonged to 7 species, including Pediococcus pentosaceus, Weissella confuse, Lactobacillus plantarum, Lactobacillus fermentum, Lactobacillus agilis, Enterococcus faecium and Lactococcus lactis. 35 strains of lactic acid bacteria were isolated from Chengdu rice wine, which belonged to 7 species, including P. pentosaceus, W. confusa, L. plantarum, E. faecium, Weissella cibaria, Lactobacillus johnsonii and Lactobacillus brevis. Among them, P. pentosaceus was the dominant strain of two kinds of rice wine, and the same species isolated from two kinds of rice wine were P. pentosaceus, W. confusa, L. plantarum and E. faecium.