西格列汀对T2DM大鼠血清神经酰胺、chemerin、胰岛功能及氧化应激指标的影响

Effects of Sig Leo Dean on serum ceramide, chemerin, islet function and oxidative stress indexes in T2DM rats

目的:探讨西格列汀对2型糖尿病(T2DM)大鼠血清神经酰胺、chemerin、胰岛功能及氧化应激指标的影响。方法:选取4周龄SPF级雄性SD大鼠54只,采用随机数字表法分为空白组、模型组、治疗组,每组18只。模型组和治疗组采用高脂高糖饲料喂养6周后,采用腹腔注射链脲佐菌素(STZ)建立T2DM大鼠模型,建模成功后治疗组给予西格列汀10 mg/kg/d,空白组和模型组均给予等量的生理盐水,持续6周后检测各项指标并进行比较。结果:干预前,模型组和治疗组大鼠的体质量、FPG、Fins、HOMA-IR显著高于空白组(P<0.05),模型组和治疗组大鼠的HOMA-β显著低于空白组(P<0.05);干预后,治疗组的FPG、Fins、HOMA-IR显著高于空白组但低于模型组(P<0.05),治疗组的体质量、HOMA-β显著高于模型组但低于空白组(P<0.05);干预前,模型组和治疗组大鼠的血清神经酰胺、chemerin显著高于空白组(P<0.05);干预后,治疗组的血清神经酰胺、chemerin显著高于空白组但低于模型组(P<0.05);干预前,模型组和治疗组大鼠的SOD、GSH-PX水平显著低于空白组(P<0.05);干预后,治疗组的SOD、GSH-PX水平显著低于空白组但高于模型组(P<0.05)。结论:西格列汀能显著改善T2DM模型大鼠的胰岛功能、降低血清神经酰胺、chemerin水平、提高大鼠抗氧化功能。

Objective: To investigate the effect of Sig Leo Dean on serum ceramide, Chemerin, islet function and oxidative stress index in type 2 diabetes mellitus (T2DM) rats. Methods: Select the SD male 4-week-old SPF 54 rats were randomly divided into blank group, model group, 18 rats in each treatment group, model group and treatment group fed with high-fat diet for 6 weeks after intraperitoneal injection of streptozotocin (STZ) to establish the rat model of T2DM. After the success of modeling in treatment group were treated with Sig Leo Dean 10 mg·kg - 1·d - 1, saline control group and model group were given equivalent, were compared after 6 weeks and the indicators of detection. Results: Before the intervention, the model group and treatment group rat body weight, FPG, Fins and HOMA-IR were significantly higher than the blank group ( P < 0.05), model group and HOMA- beta treated rats were significantly lower than that of the control group ( P <0.05);after the intervention, FPG, Fins and HOMA-IR were significantly higher than the control group in the treatment group but compared with model group ( P <0.05), treatment group, body weight, HOMA- beta is significantly higher than model group but lower than that of the control group ( P <0.05);before the intervention, the model group and the serum ceramide, the rats in the treatment group Chemerin was significantly higher than that of control group ( P <0.05);intervention, serum ceramide, Chemerin significantly higher than the control group the treatment group but lower than that of model group ( P <0.05);before the intervention, the model group and treatment group rats, the SOD level of GSH-PX was significantly lower than that of the control group ( P <0.05);intervention treatment group SOD and GSH-PX Significantly lower than the blank group, but higher than the model group ( P <0.05). Conclusion:Western medicine can significantly improve the islet function of T2DM model rats, reduce serum ceramide and Chemerin levels, and improve the antioxidant function of rats.