红树莓根茎煎剂对S180模型小鼠细胞因子水平和胸腺、脾指数的影响

Effects of raspberry root decoction on cytokine level and thymus and spleen index in S180 model mice

目的:观测红树莓根茎煎剂(RRD)对S180模型小鼠血清细胞因子白细胞介素-2(IL-2)、白细胞介素-10(IL-10)水平和胸腺、脾脏指数的影响,探究肿瘤被RRD抑制的机理。方法:选50只昆明健康小鼠,雌雄各半,按随机原则分5组,正常对照组、模型对照组、环磷酰胺组(CTX组)、红树莓组(RRD组)及红树莓和环磷酰胺联合用药组(RRD+CTX组),每组10只。除正常对照组外,其余40只小鼠在右腋窝处注射0.2 mL S180瘤株混悬液做造模实验。第二天给小鼠灌胃,正常对照组和模型对照组:0.02 mL·g^(-1)·d^(-1)生理盐水灌胃;CTX组:0.4 mL·20 g^(-1)·d^(-1)生理盐水灌胃,20 mg·kg^(-1)·d^(-1) CTX腹腔注射;RRD组:0.4 mL·20 g^(-1)·d^(-1) RRD灌胃;RRD+CTX组:0.4 mL·20 g^(-1)·d^(-1)RRD灌胃,20 mg·kg^(-1)·d^(-1) CTX腹腔注射,连续10 d,1次/d。用酶联免疫吸附法测量血清IL-2、IL-10水平;测定胸腺、脾脏指数。结果:红树莓根茎水煎剂能使S180模型小鼠血清IL-2水平升高(P<0.05), IL-10水平下降(P<0.05);还能提升S180模型小鼠胸腺指数(P<0.05)、降低脾脏指数(P<0.05)。结论:红树莓根茎水煎剂的抑瘤作用很可能和调节机体的免疫抑制状态和提高荷瘤机体的免疫器官功能有关。

Objective: To observe the effects of RRD on serum levels of cytokines interleukin-2 (IL-2), interleukin-10 (IL-10) and thymus and spleen index in S180 mice, and to explore the mechanism of tumor inhibition by RRD. Methods: Fifty Kunming healthy mice, half male and half female, were randomly divided into five groups: normal control group, model control group, cyclophosphamide group (CTX group), red raspberry group (RRD group) and combined administration of red raspberry and cyclophosphamide group (RRD + CTX group), with 10 mice in each group only. The other 40 mice were injected with 0.2 mL S180 tumor suspension at the right axilla to make the model experiment, except 10 mice in the normal control group. The next day, the normal control group and model control group were given intragastric administration of 0.02 mL· g^-1·d^-1 saline, CTX group was given intragastric administration of 0.4 mL·20 g^-1·d^-1 saline and 20 mg·kg^-1·d^-1 CTX, RRD group was given intragastric administration of 0.4 mL·20 g^-1·d^-1 RRD, RRD+CTX group was given intragastric administration of 0.4 mL·20 g^-1·d^-1 RRD and 20 mg·kg^-1·d^-1 CTX for 10 d, once a day. Serum levels of IL-2 and IL-10 were measured by ELISA, and thymus and spleen indexes were measured. Results: Red raspberry root decoction could increase serum IL-2 level ( P <0.05), decrease IL-10 level ( P <0.05), increase thymus index ( P <0.05) and decrease spleen index ( P < 0.05) in S180 mice. Conclusion: The anti-tumor effect of raspberry root decoctionmay be related to the regulation of immune suppression and the improvement of immune organ function of the tumor-bearing organism.