黄连提取物含药血清对MRSA毒力因子表达的影响

Effects of Serum Containing Coptis on the Genetic Expression of MRSA Virulence Factor

目的研究不同浓度黄连含药血清对耐甲氧西林金黄色葡萄球菌毒力因子的表达情况,阐明黄连对MRSA活性的抑制作用机制,为临床应用黄连治疗MRSA感染提供依据。方法选取5株MRSA临床分离株,大鼠灌胃给予黄连提取物后制备含药血清,采用与MRSA体外共培养16h,分为空白血清组、6mg·kg^-1组、12mg·kg^-1组、24mg·kg^-1组的黄连含药血清培养。采用荧光定量PCR方法检测各组MRSA株毒力相关基因葡萄球菌蛋白A(spa)、α-溶血素(hlα)mRNA相对表达水平。结果无抗菌活性的黄连含药血清与MRSA体外共培养16h,2个MRSA毒力因子spa和hla的mRNA相对表达水平平均低于对照组(P<0.01)。结论黄连可作为一种潜在的抗MRSA感染药物,可进一步开发。

OBJECTIVE To explore the effect of different concentrations of serum containing coptis on the genetic expression of methicillin-resistant Staphylococcus aureus(MRSA)virulence factor and illuminate their mechanisms,and to provide the basis for using coptis.METHODS Five MRSA clinical isolates were selected and were cultured with drug-containing serum of mouse which was prepared after intragastric administration with coptis for 16h.which were divided into blank serum(control group)and serum contained 6mg·kg^-1,12mg·kg^-1,24mg·kg^-1 of coptis(serum containing coptis with low,medium and high concentration groups).Fluorescent quantitative real-time PCR method was used to determine the relative mRNA expression levels of virulence-related genes staphylococcal protein A(spa),α-hemolysin(Hla)in various groups.RESULTS After co-culture for 16h,different concentrations of serum containing coptis without antibacterial activity were significantly decreased compared with control group(P<0.01).CONCLUSION The extracts from coptis are potentially useful in developing anti-MRSA drugs.