复方中药治疗母猪不孕及抗炎机理试验

Study on Sterility and Anti-inflammation Mechanism of Sow Treated with Compound Chinese Medicine

为研究复方中药治疗母猪不孕症的效果,试验选择临床上两个以上情期内配种不孕的经产杜洛克母猪138头,随机分为4组,1组为中药“猪孕散”组,以“猪孕散”前2 d口服100 g,第3天配种前口服150 g治疗;2组为西药洗宫组,先用0.1%高锰酸钾1 000 mL冲洗子宫,再用青霉素240万单位、链霉素100万单位、生理盐水100 mL,混合后注入不孕母猪子宫内,每日1次,连用3 d;3组为“猪孕散”配合洗宫组,同时使用1、2组中药加西药治疗;4组为阳性对照(不治疗)组,不添加任何药物,进行母猪不孕治疗的对照试验。选(20±2)g小白鼠40只,随机分为4组,雌雄各半,1~3组为药物高、中、低剂量试验组,分别按0.3 mL/10 g、0.2 mL/10 g和0.1 mL/10 g体重,灌服中药“猪孕散”药液;4组为空白对照组,小白鼠按0.3 mL/10 g体重灌服生理盐水,进行中药“猪孕散”抑制乙酸所致的小白鼠炎性渗出试验。结果表明:复方中药“猪孕散”治疗不孕母猪平均受胎率可达88.5%,比阳性对照组高79.5个百分点(P<0.01),比西药治疗组高67.5个百分点(P<0.01);而且复方中药“猪孕散”能够显著抑制乙酸所致的小鼠腹膜的急性炎性渗出,药物高、中剂量组小鼠腹水中伊文思蓝含量均极显著低于空白对照组(P<0.01);说明复方中药“猪孕散”治疗母猪不孕、提高受胎率有良好效果,抗炎机理与拮抗炎性渗出、促进子宫黏膜吸收有关。

To study the effect of compound Chinese medicine on sow infertility,total of 138 pregnant duroc sows were randomly divided into 4 groups:the experimental group(1-3 groups),the positive control group(no treatment)group(4 groups).In the first group,the traditional Chinese medicine"pig pregnancy powder"group was treated with 100 g oral administration two days before"pig pregnancy powder"and 150 g oral treatment on the third day before mating.In the 2 groups of western medicine uterine washing group,the uterus was first washed with 1 000 mL of 0.1%potassium permanganate,followed by 2 400 000 units of penicillin,1 000 000 units of streptomycin and 100 mL of normal saline,the mixture was injected into the uterus of infertile sows once a day for 3 days.3 groups of"pig pregnancy powder"with the uterine lavage group,at the same time using 1,2 groups of traditional Chinese medicine and western medicine treatment,4 groups of positive control(no treatment)group,do not add any drugs,sow infertility treatment trial.Forty(20±2)g mice were selected and randomly divided into four groups,half male and half female.Groups 1-3 were high-dose,medium-dose and low-dose experimental groups.According to the body weight of 0.3 mL/10 g,0.2 mL/10 g and 0.1 mL/10 g,the solution of traditional Chinese medicine"pig pregnancy powder"was administered.Four groups of blank control group,mice at the weight of 0.3 mL/10 g oral administration of normal saline,Chinese medicine"pig pregnancy powder"to inhibit the acetic acid-induced inflammatory exudation of mice test.The results showed that the average conception rate of infertile sows treated with compound Chinese medicine"pig pregnancy powder"was up to 88.5%,79.5 percent points higher than that of the positive control group(P<0.01),and 67.5 percent points higher than that of the western medicine treatment group(P<0.01).Moreover,the compound Chinese medicine"pig pregnancy powder"can significantly inhibit the acute inflammatory exudation of mouse peritoneum caused by acetic acid.The content of evanthran in the water was significantly lower than that in the control group(P<0.01).The results indicated that the compound Chinese medicine"pig pregnancy powder"had a good effect in treating sow sterility and improving the pregnancy rate,and the anti-inflammatory mechanism was related to the antagonistic inflammatory exudation and promoting the absorption of uterine mucosa.