响应面法考察六堡茶水溶性蛋白溶出特征

Study on Dissolution Characteristics of Water-soluble Protein in Liupao Tea Based on Response Surface Methodology

六堡茶含丰富的水溶性蛋白,泡饮过程中溶出被人体摄入。不同浸泡条件蛋白质溶出不同。通过料液比、浸泡时间、浸泡温度单因素和响应面分析法考察,考马斯亮蓝法测定茶蛋白含量,依据Box-Benhnken中心组合实验与响应面分析,对二次多项式回归方程模型模拟,得出六堡茶水溶性蛋白溶出最佳条件为:料液比30:1,浸泡时间2h,浸泡温度40℃。运用响应面法可确定最佳溶出条件,为六堡茶的进一步利用提供理论依据。

Liupao Tea contains more water-soluble protein,which is dissolved in the process of soaking and absorbed by human body.The amount of protein varies with the soaking conditions.The Single factor test methods about ratio of material to liquid,soaking time,soaking temperature and response surface analysis were used to optimize conditions of extracting water-soluble tea protein.And Coomassie brilliant blue method was applied to determine,the content of tea protein based on Box-Benhnken central combination test and response surface analysis,the prediction model of quadratic polynomial regression equation was simulated.the ratio of liquid to solid was 30:1,and extraction time was 2 h,and extraction time was 40℃Celsius.Response surface methodology is an effective way to optimize the protein extraction factors.