黑玛丽精子包特性及pH和温度对其精子活力的影响

Spermatophore Characters and Effects of pH and Temperature on Sperm Motility of Poecilia latipinna

通过研究黑玛丽Poecilia latipinna精子包破裂的过程和影响因素、精子运动的情况及影响因素,结果发现,当精液用Hank’s平衡盐溶液(HBSS)稀释约5 min后精子包开始破裂,约12 min后全部破裂。释放出的精子暂时处于休眠状态,约50 min后,处于HBSS稀释液中的精子会被激活。应用计算机辅助精子分析系统对黑玛丽精子在不同pH和不同温度下HBSS中的精子运动百分数、运动时间和平均运动速率进行观察。在pH7~8的中性或弱碱性溶液中,精子运动活力较强;而在酸性(pH<7)或碱性较强(pH>9)的溶液中,精子的运动活力都会降低。在不同温度下的HBSS中精子的活力不同,精子在低温(4℃)条件下的运动时间显著长于在室温(20℃)条件下,但运动速度较慢。本研究初步探讨了黑玛丽的精子包特性以及pH和温度对精子运动活力的影响,旨在对黑玛丽等卵胎生鱼类的人工授精,以及生殖生物学特性等的研究提供更丰富的基础资料。

By using Poecilia latipinna as experimental material,the process of spermatophore breakdown and the factors affecting the process,sperm motility and the factors influencing sperm motility were studied in this study.We found that the spermatophore began to break at about 5 min and completely ruptured at about 12 min after the semen was diluted with Hank’s balanced salt solution(HBSS).In addition,we also observed that all the sperms released from the broken spermatophore were temporarily dormant,and the sperms in HBSS diluent were activated after about 50 min.The percentage of swimming sperm,swimming duration and velocity of the sperm were also studied by a computer-assisted semen analyser in HBSS at different pH and temperatures.The results showed that neutral and weak alkaline solutions with pH from 7 to 8 were optimum for sperm swimming(or motility),while acidic solutions with pH<7 or strong alkaline solutions with pH>9 both reduced sperm motility.The total motility span of sperm was significantly longer,and the sperm swimming velocity was significantly slower when kept at low temperature(4℃)compared with those at room temperature(20℃).Therefore,these findings provide valuable references for artificial insemination,and for the reproductive biological knowledge of these fish.