染料木素对APAP诱导小鼠肝纤维化的影响

Effects of Genistein on APAP-induced Fibrosis of Liver Injury in Mice

目的:研究染料木素对对乙酰氨基酚(APAP)诱导小鼠肝纤维化的保护作用。方法:将40只雄性BALB/c小鼠随机分为正常对照组、模型组、染料木素低(50 mg/kg)、高剂量组(100 mg/kg),每组10只。采用灌胃APAP建立小鼠肝损伤肝纤维化模型,观察染料木素药物干预后的影响。应用HE染色和Masson染色检测肝脏组织病理学变化;TUNEL法检测肝细胞凋亡情况;免疫组织化学染色检测结缔组织生长因子(CTGF)、肿瘤坏死因子-α(TNF-α),白介素6(IL-6)的表达。结果:HE和Masson染色结果显示,染料木素可明显减轻模型小鼠肝脏炎性损伤及坏死,减少胶原纤维沉积;TUNEL染色结果显示,染料木素组小鼠肝细胞凋亡数量显著低于模型组(P<0.01);免疫组织化学染色结果显示,染料木素组小鼠肝脏中CTGF、TNF-α、IL-6表达显著低于模型组(P<0.05或P<0.01)。结论:染料木素具有改善APAP诱导小鼠肝损伤和肝纤维化的作用。

Objective:To investigate the protective effect of genistein on acetaminophen(APAP)-induced fibrosis of liver injury in mice.Methods:Forty male BALB/c mice were randomly divided into control group,model group and low-(50 mg/kg),high-dose genistein group(100 mg/kg),each group was 10 mice.The fibrosis model of liver injury in mice was established by intragastric administration of APAP to observe the effect of genistein drug intervention.Histopathological changes of the liver were detected by HE staining and Masson staining.Apoptosis of hepatocytes was detected by TUNEL assay;The expressions of connective tissue growth factor(CTGF),tumor necrosis factor-α(TNF-α)and interleukin 6(IL-6)were detected by immunohistochemical staining.Results:HE and Masson staining results showed that the genistein group could significantly reduce liver inflammatory injury and necrosis and reduce collagen fiber deposition of the model mice.TUNEL staining showed that the number of hepatocyte apoptosis in the genistein group was significantly lower than that in the model group(P<0.01).Immunohistochemical staining showed that the expressions of CTGF,TNF-α and IL-6 in the liver of genistein group were significantly lower than that in the model group(P<0.05 or P<0.01).Conclusion:Genistein has the effect of improving APAP-induced liver damage and hepatic fibrosis in mice.